摘要
从土壤中分离到木霉菌株Tvir-6,结合形态学和分子生物学的ITS和tef1序列扩增法鉴定该木霉菌株为绿色木霉(Trichoderma viride)。通过发酵液试验、盆栽和田间试验分析了绿色木霉菌株Tvir-6对根结线虫的防治效果,结果表明,绿色木霉菌株Tvir-6发酵液在24 h内对根结线虫二龄幼虫的校正死亡率高达98.5%,在盆栽试验中根结减退率为78.2%,在田间试验中防效达到64.9%,与10%噻唑膦颗粒剂防治效果相近,并可使黄瓜产量提高34.1%。绿色木霉菌株Tvir-6的筛选及作用效果研究丰富了黄瓜根结线虫生防菌资源,具有重要应用潜力,为防治黄瓜根结线虫提供了重要材料及依据。
In this study,the strain Tvir-6was isolated from soil in vegetable field,combining morphologicaland molecular biology of ITS and tef1sequence amplification method,strain Tvir-6was identified as Trichodermaviride.The biocontrol effect of T.viride Tvir-6on cucumber root-knot nematode was evaluated via fermentationbroth test,pot and field experiments.The results indicated that the corrected mortality rate of T.viride Tvir-6fermentation broth to Meloidogyne incognita J2was as high as98.5%,the biocontrol effect was78.2%in potexperiment,and64.9%in field test,showing T.viride Tvir-6had a similar biocontrol effect with10%Fosthiazate.At the same time,T.viride Tvir-6could increase cucumber yield up to34.1%in field experiment.Studies on theeffect of T.viride Tvir-6and screening for it had enriched the biocontrol bacterium resources for cucumber rootknotnematode.This study had a great potential in application and provided an important material and basis forbiocontrol against root-knot nematodes on cucumber.
作者
翟明娟
李登辉
马玉琴
朱萍萍
茆振川
杨宇红
谢丙炎
ZHAI Ming-juan;LI Deng-hui;MA Yu-qin;ZHU Ping-ping;MAO Zhen-chuan;YANG Yu-hong;XIE Bing-yan(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Key Laboratory of Horticultural Crops Biology and Genetic Improvement,Ministry of Agriculture,Beijing 100081,China)
出处
《中国蔬菜》
北大核心
2017年第10期67-72,共6页
China Vegetables
基金
国家重点研发计划项目(2016YFD0201000)
关键词
绿色木霉
黄瓜
南方根结线虫
防治效果
Trichoderma viride
Cucumber
Meloidogyne incognita
Control effect
