摘要
采用桶栽方式,对抗旱性强的F172和抗旱性弱的YL6甘蔗品种在苗期进行重度干旱胁迫处理后,应用蛋白质双向电泳技术进行差异蛋白质分析,分别找出差异显著的28和20个差异蛋白点,其中部分呈现上调表达,部分呈现下调表达,还有部分新增的蛋白点,因品种抗性不同而表现各异,F172叶片中的差异蛋白主要表现为上调表达,而YL6中大多表现为下调表达。在重度干旱胁迫下,抗旱性不同的甘蔗品种蛋白质丰度变化有显著差异。采用MALDI-TOF-TOF/MS鉴定所获得的差异蛋白,从YL6、F172中分别鉴定出18、14个蛋白的氨基酸序列,对所鉴定的蛋白质根据功能分为8类。YL6中参与自由基清除的2个,参与光合作用的6个,参与细胞生长和分裂的1个,参与基础代谢的6个,参与防卫反应的2个,未知功能蛋白1个。F172中参与自由基清除的1个,参与光合作用的2个,参与细胞生长和分裂的2个,参与基础代谢的4个,参与信号转导的2个,参与蛋白加工的1个,未知功能蛋白2个,其中22 k D干旱诱导蛋白的丰度明显提高,而在YL6中则检测不到此蛋白。这说明在干旱胁迫下抗旱性不同的甘蔗品种在蛋白质组成上有很大差异,推测这是不同甘蔗品种间抗旱性差异的重要分子基础。
Drought stress is a major restraint in sugarcane production in China.Proteomic study in relation to drought stress provides valuable information in drought resistant breeding of sugarcane.In this study,the drought-resistant sugarcane variety,F172,and the drought-sensitive variety,YL6,were used in a pot experiment for differential proteome analysis.Seedlings of both varieties were exposed to severe drought stress for seven days and the leaf proteins were separated and analyzed using2-DE technique and PDQuest software.From the protein profiles of F172and YL6,28and20differential protein spots were detected between normal-irrigation and drought-stress treatments,respectively,including up-and down-regulated proteins and new protein spots.The differential proteins varied across the two varieties.Using MALDI-TOF-TOF/MS,18and14amino acid sequences were identified from YL6and F172,respectively,and they were in eight function categories.In YL6,the18proteins consist of two participating in oxygen radical scavenging,six participating in photosynthesis,one participating in cell growth and division,six participating in basic metabolisms,two participating in protective response,and one unknown in function.In F172,the14proteins consist of one participating in oxygen radical scavenging,two participating in photosynthesis,two participating in cell growth and division,four participating in basic metabolisms,two participating in information transfer,one participating in protein processing,and one unknown in function.A drought-induced protein of22kDa was in high level in F172but absence in YL6.These results indicate that protein compositions under drought stress are highly different in sugarcane varieties with different drought resistance and the differential proteins might give a hint to drought-resistant mechanism.
作者
DO Thanh-Trung
李健
张风娟
杨丽涛
李杨瑞
邢永秀
DO Thanh-Trung;LI Jian;ZHANG Feng-Juan;YANG Li-Tao;LI Yang-Rui;XING Yong-Xiu(Agricultural College, State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Nanning 530005, China;Guangxi Academy of Agricultural Sciences / Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Guangxi Key Laboratory of Sugarcane Biotechnology and Genetic Improvement, Ministry of Agriculture / Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007, China)
出处
《作物学报》
CAS
CSCD
北大核心
2017年第9期1337-1346,共10页
Acta Agronomica Sinica
基金
国家高技术研究发展计划项目(863计划)(2013AA102604)
广西八桂学者
特聘专家专项(2013)
国家现代农业产业技术体系广西甘蔗创新团队专项(gjnytxgxcxtd-03-01)
广西甘蔗遗传改良重点实验室项目(12-K-05-01)资助~~
关键词
甘蔗
水分胁迫
蛋白质组
差异表达
抗旱性
Sugarcane
Water stress
Proteome
Differential expression
Drought resistance
