摘要
目的探讨从龙牙楤木总皂苷中分离得到的楤木皂苷C(aralosdie C,SMC)对缺血/再灌注(ischemia/reperfusion,I/R)损伤的大鼠心肌细胞收缩功能和钙瞬变的影响,及钙调蛋白激酶Ⅱ(Ca MKⅡ)在其中发挥的作用。方法采用Langendorff心脏灌流系统分离原代大鼠心肌细胞,分为对照组(Control组)、模型组(I/R组)、楤木皂苷C组(I/R+SMC8μmol·L^(-1))、抑制剂组(I/R+KN-93 1μmol·L^(-1)+SMC 8μmol·L^(-1)),采用细胞收缩与离子浓度同步测定系统同步检测SMC通过调控Ca MKⅡ对单个I/R细胞收缩功能和钙瞬变的影响。结果 8μmol·L^(-1)SMC作用5 min和未加入SMC的I/R成年大鼠心肌细胞收缩功能进行比较,发现SMC处理可明显促进I/R成年大鼠心肌细胞收缩功能的恢复,SMC改善心肌细胞收缩的同时,其钙瞬变也相应改善,并且SMC使钙瞬变幅度和[Ca2+]i上升/下降的速率明显升高,但加入KN-93抑制Ca MKⅡ后,除稳态钙息值无变化外,SMC对钙瞬变的作用效果降低。结论 SMC可明显改善I/R成年大鼠心肌细胞的收缩功能和钙瞬变,其作用机制可能与Ca MKⅡ有关。
AimTo investigate the effects of aralosdie C(SMC)isolated from aralosides on contractile function and calcium transients in ischemia/reperfusion(I/R)induced rat myocardial injury and the role of CaMK II.MethodsThe cardiomyocytes were divided into control group,I/R group,I/R+SMC group,KN93+I/R+SMC group.The effect of SMC on the contractile function and calcium transient of I/R cells was measured by cell shrinkage and ion concentration simultaneous detection system.ResultsSMC(8μmol·L-1)improved the contractile function of I/R cardiomyocytes and the calcium transients,and SMC increased the rate of calcium transient and[Ca2+]i up/down regulation significantly.While CaMK II was blocked by KN93,the effect of SMC on contractile function and calcium transients was weakened.ConclusionsSMC can significantly improve the systolic function and calcium transients of cardiomyocytes in I/R rats,and the protective effect of SMC on cardiomyocytes may be related to CaMK II.
作者
季宇彬
刘雪松
张妙笛
王敏
张静宜
王瑞莹
孙桂波
孙晓波
JI Yu bin;LIU Xue song;ZHANG Miao di;WANG Min;ZHANG Jing yi;WANG Rui ying;SUN Gui bo;SUN Xiao bo(Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin150076, China;Pharmacology and Toxicology Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing100193, China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2017年第12期1678-1684,共7页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81473380)
北京市自然科学基金资助项目(No 7152102)
关键词
楤木皂苷C
心肌细胞
CaMKⅡ
缺血/再灌注
细胞收缩与离子浓度同步测定系统
细胞收缩和钙瞬变
aralosdie C(SMC)
cardiomyocyte
CaMK II
ischemia/reperfusion
cell contraction and ion concentration synchronization determination system
cell contraction and calcium transient
