摘要
目的比较无血清条件下不同组织块法培养人角膜上皮细胞的出膜时间及出膜率。方法培养方式:A组:剥除角膜内皮及刮除角膜上皮后组织块角膜上皮面向上贴壁;B组:剥除角膜内皮及刮除角膜上皮后组织块角膜上皮面向下贴壁;C组:仅剥除角膜内皮后全层组织块角膜上皮面向上贴壁;D组:仅剥除角膜内皮后全层组织块角膜上皮面向下贴壁。相差显微镜观察细胞形态学变化,对比各组出膜时间及出膜率。结果 A组:细胞爬出时间(7.00±3.00)d,出膜率47.50%(19/40);B组:细胞爬出时间(7.55±2.58)d,出膜率45.83%(11/24);C组:细胞爬出时间(8.43±3.32)d,出膜率63.64%(14/22);D组:细胞爬出时间(7.49±2.20)d,出膜率72.22%(39/54)。卡方检验结果示,A组与D组、B组与D组出膜率相比,差异均有统计学意义(均为P<0.05),余组间两两比较差异均无统计学意义(均为P>0.05)。结论无血清条件下不同组织块培养方式细胞爬出时间大致相同,约一周。相比较而言,仅剥除角膜内皮后全层组织块角膜上皮面向下贴壁培养法出膜率明显较高,且培养的细胞更纯、更稳定。
ObjectiveTo compare and analyze the time of cell eruption formation and the rate of cell eruption of human corneal epithelial cells cultured by different explant culture methods under serum free conditions.MethodsExplant tissues were randomly divided into A,B,C and D groups.In A group,the cornea epithelial cells were put side up after stripping corneal endothelium and scraping the corneal epithelium;in B group,the corneal epithelium was put side down after stripping corneal endothelium and scraping the corneal epithelium;in C group,the corneal epithelium was put side up after only stripping corneal endothelium;and in D group,the corneal epithelium was put side down after only stripping corneal endothelium.The morphological changes of the cells in the4groups were observed by phase contrast microscope,and both aforementioned variable were recorded.ResultsThe average time of cell eruption formation and the rate of cell eruption in A,B,C and D group was(7.00±3.00)d and47.50%(19/40),(7.55±2.58)d and45.83%(11/24),(8.43±3.32)d and63.64%(14/22),(7.49±2.20)d and72.22%(39/54),respectively.The chi square test showed that there was significant difference in the cell eruption rate between A and D group as well as B and D group(all P<0.05),but there was no significant difference between the other groups(all P>0.05).ConclusionThe time of cell eruption formation in the four different explant culture methods was about one week in free serum conditions.And D group has higher cell eruption rate,and pure plus stable cultured cells when compared with the other three groups.
作者
史小玲
王骞
鹿晓燕
SHI Xiao Ling;WANG Qian;LU Xiao Yan(the Affiliated Xiamen Eye Center & Eye Institute of Xiamen University Xiamen 361001,Fujian Province,China;Department of Ophthalmology,the First Affiliated Hospital of Zhengzhou University Zhengzhou 450052,Henan Province,China)
出处
《眼科新进展》
CAS
北大核心
2017年第12期1132-1134,共3页
Recent Advances in Ophthalmology
关键词
角膜上皮细胞
组织块法
无血清
细胞培养
corneal epithelial cell
explant culture
serum free
cell culture
