摘要
采用悬浮聚合法,以奥硝唑(ONZ)为模板分子、甲基丙烯酸(MAA)和丙烯酰胺(AA)为双功能单体、二甲基丙烯酸乙二醇酯(EDMA)为交联剂,利用Fe_3O_4磁性纳米粒子制备了奥硝唑磁性分子印迹聚合物(MMIPs)。通过扫描电子显微镜(SEM)、红外光谱(FT-IR)、磁化强度分析(VSM)、紫外-可见吸收光谱(UV-Vis)及热重分析(TGA)等对合成的聚合物进行了表征。结果表明,双功能单体MMIPs对奥硝唑的吸附量高于相应的单功能单体MMIPs;对类似物质也有一定的吸附能力。利用等温吸附数据进行Scatchard分析,从而推断出MMIPs对于模板分子的作用存在两种不同的结合位点,最大吸附量Q_(max1)为48.96μmol/g,Q_(max2)为10.60μmol/g。结合高效液相色谱分析技术,将所制备的MMIPs应用于河水样品中硝基咪唑类抗生素的提取富集,加标回收率为85.4%~104.3%,方法简便易行、准确可靠。
The magnetic molecularly imprinted polymers(MMIPs)was synthesized by suspension polymers technique using ornidazole(ONZ)as template,and methacrylic acid(MAA)and acrylamide(AA)as the binary functional monomers.The MMIPs were characterized by means of scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FT-IR),vibrating sample magnetometer(VSM),thermogravimetric analysis(TGA)and ultraviolet-visible(UV-Vis)spectrophotometer.The results indicated that the adsorption capacity of MMIPs using the binary functional monomers to ONZ was higher than that using single functional monomer or that of its structural analogues.The Scatchard plot revealed that the template polymer system had a two-site binding behavior and the MMIPs exhibited the maximum rebinding to ONZ at48.96umol/g and10.60umol/g.Combined with high performance liquid chromatographic analysis technology,the prepared MMIPs were successfully applied to extract and enrich trace nitroimidazoles from river water samples with recoveries range of85.4%-104.3%.The precision of the results was good,and the manipulation of the developed method was simple and fast comparing to other methods.
作者
廖素兰
陈少云
刘奇琳
陈良壁
李晓敏
LIAO Su-Lan;CHEN Shao-Yun;LIU Qi-Lin;CHEN Liang-Bi;LI Xiao-Min(College of Tea and Food Science, Wuyi University, Wuyishan 354300, China)
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2018年第1期100-106,共7页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.21407118)
福建省高等学校新世纪优秀人才支持计划(No.闽教科[2016]23号)
福建省科技计划重点项目(No.2015N0035)
武夷学院科技项目(No.XL201303)资助~~
关键词
双功能单体
磁性分子印迹
硝基咪唑类药物
高效液相色谱
固相萃取
Dual functional Monomers
Magnetic molecularly imprinted
Nitroimidazoles
High performance liquid chromatography
Solid phase extraction
