摘要
目的探索高频电磁辐射造成小鼠情绪-认知行为损伤模型的建立方法,为辐射损伤机制及防护研究提供实验基础。方法高频电磁混响室可产生持续而均匀的电磁辐射波,小鼠分别暴露于高频电磁辐射箱1 d、3 d和7 d,进行系列行为学检测,观察辐射组小鼠在自主活动、焦虑样行为、抑郁样行为以及认知功能的改变。结果高频电磁辐射(频率3 GHz,SAR值为4 W/kg)暴露每天4 h,连续暴露7 d,不影响小鼠的自主活动,却可导致辐射组小鼠进入开场中央区的次数(P<0.05)和时间(P<0.05)显著减少;与未暴露组相比,辐射组小鼠显著降低在高架十字迷宫实验中进入开臂的次数(P<0.05)和时间百分比(P<0.01);同时此剂量的电磁辐射暴露也显著增加小鼠在强迫游泳和悬尾实验中的不动时间(P<0.01,P<0.05);并且辐射暴露组比未暴露组小鼠显著降低在新物体识别实验中对新物体的区分指数(P<0.001)。结论持续高频电磁辐射(频率为3 GHz,SAR值为4 W/kg)可时间依赖地导致小鼠焦虑样-抑郁样行为以及认知功能损伤,这为相关防治技术的研究提供了实验依据。
Objective To explore the effects of high-frequency electromagnetic radiation exposure on the mood-cognitive behaviors of mice,and to provide an experimental basis for the study on radiation damage mechanism and protection.Methods The electromagnetic reverberation chamber can produce continuous and uniform high-frequency electromagnetic waves.Different groups of ICR mice were exposed to high-frequency electromagnetic radiation in this chamber for 1 d,3 d and 7 d,and then series of behavioral tests were conducted to observe the changes in locomotor activities,depressive-like and anxiety-like behaviors,and cognitive function of the mice.Results After exposed to the high-frequency electromagnetic radiation(3 GHz,SAR 4 W/kg)4 h/d for 7 d,there was no obvious change in locomotor activity(times of grid crossing and standing up in the open field test)of the mice,while the times of entering the central region(P<0.05)and the time duration(P<0.05)were significantly decreased.Compared with the unexposed group,the percentage of the times of entering the open arm in the elevated-plus maze(P<0.05)and the time duration in the open arm(P<0.01)of the mice in the radiation group were significantly decreased.Meanwhile,the immobility time in the forced-swimming test(P<0.01)and the tail suspension test(P<0.05)of the mice exposed to electromagnetic radiation was significantly increased,and the discrimination index of new objects in the new object recognition test was significantly reduced(P<0.001).Conclusions Continual exposure to high-frequency electromagnetic radiation(3 GHz,SAR 4 W/kg)can lead to anxious-depression behaviors and cognitive impairment of mice in a time-dependent manner,which provides an experimental basis for the relevant study of prevention and therapy techniques.
作者
孙立君
高艳
郭颖
张成岗
张有志
李云峰
SUN Lijun;GAO Yan;GUO Ying;ZHANG Chenggang;ZHANG Youzhi;LI Yunfeng(Beijing Key Laboratory of Neuropsychopharmacology,Beijing Institute of Pharmacology and Toxicology,Beijing 100850,China;Beijing Institute of Radiation Medicine,Beijing 100850;General Hospital of PLA, Beijing 100853)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第3期15-20,共6页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金项目(编号:81373035)。
关键词
电磁辐射
抑郁
焦虑
认知损伤
行为学检测
electromagnetic radiation
depression
anxiety
cognitive impairment
behavioral tests
