摘要
研究姜黄挥发油(TVO)对人皮肤鳞状细胞癌SCL-1细胞体外增殖、迁移与侵袭的影响,初步探讨其凋亡作用机制。运用MTT法检测不同浓度TVO在不同时间段(24、48、72h)对SCL-1细胞体外增殖的影响;细胞划痕和细胞侵袭实验检测TVO对皮肤鳞癌SCL-1细胞体外迁移和侵袭能力的影响;流式细胞术分析及显微镜观察确定TVO诱导SCL-1细胞的诱导凋亡率;Western blot检测TVO对皮肤鳞癌SCL-1细胞Survivin及Caspase-3蛋白表达的影响。TVO对SCL-1细胞的体外增殖、迁移和侵袭具有抑制作用(P<0.05),且呈时间-剂量依赖性;流式细胞仪分析及显微镜观察表明TVO可诱导SCL-1细胞凋亡(P<0.05),且与其浓度呈正相关;Western blot检测结果表明TVO能显著上调凋亡相关蛋白Caspase-3的表达(P<0.05)、下调Survivin蛋白的表达(P<0.05)。TVO能够显著抑制人皮肤鳞癌SCL-1细胞的体外增殖、迁移与侵袭能力,且诱导细胞凋亡,其可能的机制是通过调节凋亡相关蛋白survivin、caspase-3蛋白的表达。
To study the effect of turmeric volatile oil(TVO)on the proliferation,migration and invasion of human skin squamous cell carcinoma cell line SCL-1 in vitro,and to explore the mechanism of its apoptosis.The effects of different concentrations and time(24,48,72 h)on the proliferation of SCL-1 cells were detected by MTT;The effects of TVO on migration and invasion of skin squamous cell carcinoma SCL-1 cells were examined by Wound scratch assay and Transwell;Flow cytometry analysis and microscope to determine the apoptosis rate of SCL-1 cells induced by TVO;Western Blot detects Survivin and Caspase-3 protein expression.TVO has inhibitory on the proliferation,migration and invasion of SCL-1 cells(P<0.05)in a time-dose dependent relationship.Flow cytometry analysis showed that TVO can induce apoptosis of SCL-1 cells(P<0.05);The flower-cell analysis and microscopic observation showed that TVO can induce the apoptosis of SCL-1,which was directly related to its concentration;Western blot test results showed that TVO can significantly increase the expression of apoptosis related Caspase-3(P<0.05),lower the expression of Survivin protein(P<0.05).TVO can significantly inhibit human skin squamous carcinoma SCL-1 cell apoptosis induced by in vitro proliferation,migration,invasion ability and its possible mechanism is by regulating apoptosis related proteins expression of survivin,caspase-3 protein.
作者
吕琳娜
昝雪娟
荣冬芸
肖露
骆衡
曹煜
LV Lin-na;ZAN Xue-juan;RONG Dong-yun;XIAO Lu;LUO Heng;CAO Yu(Guizhou Medical University,Guizhou 550004,China;Department of Dermatology;Xingyi People's Hospital of Guizhou,Yingyi 562400,China;State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550014,China;Key Laboratory of Chemistry for Natural Products of Guizhou Province,Chinese Academy of Sciences,Guiyang 550014,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2018年第3期379-384,361,共7页
Natural Product Research and Development
基金
贵州省中药现代化专项项目[2012-5018]
贵州省中药民族药创制工程中心[2012-018]
