摘要
目的研究抑制信号传导和转录激活因子1(STAT1)基因表达对胃癌MKN-45细胞增殖、侵袭、迁移等表观遗传学特性的影响。方法将胃癌细胞分为三组:空白对照组不做任何处理,空载体对照组、STAT1 SiRNA处理组分别转染SiRNA和特异性干扰STAT1基因表达的STAT1 SiRNA。蛋白质印迹法(Western Blot)检测STAT1、血管内皮生长因子(VEGF)、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)蛋白的表达;噻唑蓝(MTT)法检测细胞的增殖活性;Transwell法检测MKN-45细胞侵袭、迁移能力。结果抑制STAT1表达后,MKN-45细胞的增殖活性、侵袭以及迁移能力显著降低(P<0.05),且STAT1 SiRNA处理组细胞中VEGF、MMP-2、MMP-9表达量较空白对照组明显降低(P<0.05);空载体对照组与空白对照组细胞的增殖活性、侵袭以及迁移能力、VEGF、MMP-9、MMP-2表达量比较差异均无显著性(P>0.05)。结论 STAT1可能通过调控肿瘤细胞的转移能力以及血管生成等抑制胃癌细胞增殖及侵袭、迁移能力。
Objective To investigate the effects of inhibiting the expression of Signal transducer and activator of transcription-1(STAT1)gene on the proliferation,invasion and migration of gastric cancer MKN-45 cells.Methods The gastric cancer cells were divided into blank control group,empty control group,STAT1 SiRNA group;Transfection of SiRNA and STAT1 siRNA specifically interfered with the expression of STAT1 gene.The expression of STAT1,VEGF,MMP-2 and MMP-9 were detected by Western Blot.The cell proliferation activity was measured by MTT assay.Transwell assay was used to detect the invasion and migration of MKN-45 cells.Results After inhibited the expression of STAT1,the proliferation,invasion and migration of MKN-45 cells were significantly decreased(P<0.05),and the expression of VEGF,MMP-2 and MMP-9 in STAT1 siRNA group were significantly lower than those in the blank control group(P<0.05).The proliferation activity,invasion and migration ability,the expression of VEGF,MMP-9,MMP-2 in empty vector control group and blank control group had no significant difference(P>0.05).Conclusion STAT1 may inhibit the proliferation,invasion and migration of gastric cancer cells by the regulation of tumor cell metastasis and angiogenesis.
作者
刘欢
谭新华
LIU Huan;TAN Xin-hua(Minimally Invasive Surgery,The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha Hunan 410007,China)
出处
《临床和实验医学杂志》
2018年第6期580-583,共4页
Journal of Clinical and Experimental Medicine
