摘要
脱氢醋酸钠作为防霉剂已广泛应用于食品、饲料及化妆品等多个领域,但有关脱氢醋酸钠使用后,引起动物组织出血的报道极少。通过灌胃给予Wistar大鼠50、100、150、200mg/kg脱氢醋酸钠,连续12d,对照组灌胃生理盐水。在灌胃后不同时间测定大鼠体重、饲料消耗、凝血酶原时间(PT)和活化部分凝血酶原时间(APTT),并对主要脏器进行组织病理学观察。结果表明,150mg/kg和200mg/kg组灌胃3d后,大鼠体重和饲料消耗与对照组相比显著降低(P<0.05);50mg/kg和100mg/kg组则在7d^10d后,显著低于对照组(P<0.05)。试验期内雌鼠增重远远小于雄鼠。给予脱氢醋酸钠后,所测时间点的PT、APTT值均极显著高于正常对照组(P<0.01),雌雄大鼠间APTT值有明显差异。雌性大鼠的APTT值随剂量、时间的增加而延长。组织切片检查心、肝、脾、肺、肾存在不同程度的出血,肝和肾组织出血较明显。结果表明,较大剂量脱氢醋酸钠可致大鼠凝血功能障碍,能引起主要脏器不同程度出血的倾向。作为食品或饲料添加剂使用,使用高剂量脱氢醋酸钠时,存在一定的安全风险。
Sodium dehydroacetate is widely used in foods,feed and cosmetics etc.as an antifungal additive,but little information is focused on its anticoagulation.Wistar rats were given with a gavage of sodium dehydroacetate once a day for 12 d at doses of 50,100,150 and 200 mg/kg,and saline as a control.The body weight,feed consumption,prothrombin time(PT)and activated partial thromboplastin time(APTT)were measured at different time post administration.Histopathological analysis was mead for main tissues.The results showed that the body weight and feed consumption in 150 and 200 mg/kg at 3 d post administration were significantly lower than that in control,whereas those in 50 and 100 mg/kg at 7 d-10 d post administration were significantly lower than that in control(P<0.05).The weight gain of female rats was lower than that of male in whole test time period.The values of PT,APTT in groups treated by sodium dehydroacetiate were extremely higher than that in control at experimental period(P<0.01).There were clearly differences between female and male in APTT data,which the value of APTT in female increased with increasing of dose and exposure time of sodium dehydroacetate.Some extent hemorrages in heart,liver,spleen,lung and kidney tissues were observed in histopathological analysis.The results showed that coagulation diaorders or varying degrees of hemorrages in main organs may be induced by high dose sodium dehydroacetate,implying safety risk on human and animals when it is used as a food or feed additive.
作者
余峥嵘
吴银超
谢佳玉
谢阳
李楠
鲁倩倩
张雨梅
YU Zheng-rong;WU Yi-chao;XIE Jia-yu;XIE Yang;LI Lan;LU Qian-qian;ZHANG Yu-mei(Department of Veterinary Pharmacology and Toxicology,College of Veterinary Medicine, Yangzhou University,Yangzhou,Jiangsu,225009,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou,Jiangsu,225009,China)
出处
《动物医学进展》
北大核心
2018年第1期73-78,共6页
Progress In Veterinary Medicine
基金
江苏高校优势学科建设工程项目
江苏省产学研合作前瞻性联合研究项目(BY2015062-02)
