摘要
建立采用高效液相色谱测定0.5%吡蚜酮·杀虫双缓释粒的定量分析方法。方法以乙腈+水+磷酸盐缓冲液(体积比4∶86∶10)为流动相,采用Zorbax Eclipse XDB-C18反相柱对试样中吡蚜酮和杀虫双进行分离、定量。吡蚜酮和杀虫双线性相关系数分别为0.999 9和0.999 8,标准偏差分别为0.000 8和0.001 4,变异系数分别为0.76%和0.35%,平均回收率分别为99.59%和99.23%。该方法操作简便,线性关系好,精密度与准确度高。
A method for quantitative determination of pymetrozine+bisultap 0.5%BRG by HPLC was developed.The simultaneous determination of pymetrozine and bisultap in the samples was conducted with Zorbax Eclipse XDB-C18 column,the mixture solution of acetonitrile,water and phosphate buffer(volume ratio of 4∶86∶10)as mobile phase by HPLC and UV detector at 242 nm.The linear correlation coefficients of pymetrozine and bisultap were 0.999 9 and 0.999 8,the standard deviations were 0.000 8 and 0.001 4,the variation coefficients were 0.76%and 0.35%,and the average recoveries were 99.59%and 99.23%,respectively.The method was proved to be convenient in operation with good linear relationship,high precision and accuracy,and could be applicable in the quantitative analysis of pymetrozine and bisultap mixture preparation.
作者
吴亚坚
徐赛
李飞
韩冲冲
彭文文
李保同
WU Ya-jian;XU Sai;LI Fei;HAN Chong-chong;PENG Wen-wen;LI Bao-tong(College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China)
出处
《现代农药》
CAS
2018年第1期29-31,共3页
MODERN AGROCHEMICALS
基金
"十三.五"国家重点研发计划(2017YFD0301604)
江西省科技支撑计划(20151BBF60070)
