摘要
目的建立康复新液(美洲大蠊提取物)HPLC指纹图谱,并测定6种成分含有量。方法该药物的分析采用TOSOH TSK-GEL ODS色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-水(含0.07%乙酸),梯度洗脱;体积流量1.0 mL/min;检测波长280 nm。结果 10批样品(Ⅰ~Ⅹ)指纹图谱中有24个共有峰,相似度0.932~0.993(除样品Ⅰ)。尿嘧啶、次黄嘌呤、黄嘌呤、肌苷、原儿茶酸、环(甘氨酰-酪氨酸)分别在3.460~173.0、3.960~198.0、3.596~179.8、1.338~66.9、3.672~183.6、3.552~177.6μg/mL范围内线性关系良好(r>0.999 0),平均加样回收率(RSD)分别为99.8%(2.65%)、98.0%(2.55%)、99.7%(1.59%)、100.7%(2.80%)、102.0%(2.09%)、99.6%(1.88%)。结论该方法准确、稳定、简单,可用于康复新液的质量控制。
AIM To establish the HPLC fingerprints of Kangfuxin Liquid(extract of Periplaneta americana L.)and to determine the contents of six constituents.METHODS The analysis of this drug was performed on a TOSOH TSK-GEL ODS column(250 mm x4.6 mm,5μm),with the mobile phase comprising of acetonitrile-water(containing 0.07%acetic acid)flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.RESULTS There were twenty-four common peaks in the fingerprints of ten batches of samples(I-X)with the similarities of 0.932-0.993(except for sample I).Uracil,hypoxanthine,xanthine,inosine,protocatechuic acid and Cyclo(Gly-Tyr)showed good linear relationships within the ranges of 3.460-173.0,3.960-198.0,3.596-179.8,1.338-66.9,3.672-183.6 and 3.552-177.6(μg/mL,whose average recoveries(RSDS)were 99.8%(2.65%),98.0%(2.55%),99.7%(1.59%),100.7%(2.80%),102.0%(2.09%)and 99.6%(1.88%),respectively.CONCLUSION This accurate,stable and simple method can be used for the quality control of Kangfuxin Liquid.
作者
吕娜
王钧篪
陈丽华
沈连刚
李广志
斯建勇
LU Na;WANG Jun-chi;CHEN Li-hua;SHEN Lian-gang;LI Guang-zhi;SI Jian-yong(Ministry of Education Key Laboratory for Bioactive Substances and Resources Utilization of Chinese Herbal Medicine;Institute for Medicinal Plants Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China)
出处
《中成药》
CAS
CSCD
北大核心
2018年第3期613-617,共5页
Chinese Traditional Patent Medicine
基金
“十二五”国家科技重大创新药物专项(2011ZX09102-101-01,2012ZX09301-002-001)
