摘要
生长素响应因子(ARF)是植物中可以特异性结合生长素应答基因启动子的转录因子,在植物的生长发育中起着重要的调控作用。为了更好的了解亚洲棉中ARF基因家族的种类和数量,本文利用生物信息学的方法,分析了亚洲棉基因组中ARF基因的种类、进化关系、物理定位以及基因的结构和保守基序。结果表明在亚洲棉基因组中发现并初步确定了29个ARF基因,命名为Ga ARF,通过基因定位发现除在2、3、5、11号染色体上没有ARF基因外,其他染色体上都有ARF基因存在;而且在10号染色体上还存在着基因簇;进化树分析表明,亚洲棉部分ARF蛋白和已知功能的拟南芥聚类到一起;表达模式分析显示,不同组织不同处理条件下ARF基因的表达存在差异。
Auxin response factor(ARF)is an important transcription factor that can specifically bind auxin responsive gene promoter in plants,and influences the plant growth and development.To better understand the type and quantity of ARF gene families in Gossypium arboretum,we used the method of bioinformatics to analyze the structure types physical mapping of genes and conserved motifs of genome in Gossypium arboretum.The results show that 29 ARF genes,named GaARF were identified in the genome of Gossypium arboretum.All chromosomes contain the ARF gene except the 2,3,5 and 11 by analyzing the position of genes.In addition,there is a gene cluster on chromosome 10.Phylogenetic analysis showed that some ARF proteins and proteins with known function in Arabidopsis were clustered together.Expression profile analysis indicated the expression of ARF genes were diverse in different tissues.
作者
冯庭
司国阳
李国辉
郭宁
蔡永萍
FENG Ting;SI Guoyang;LI Guohui;GUO Ning;CAI Yongping(School of Life Sciences,Anhui Agriculture University,Hefei 230036,China)
出处
《生物信息学》
2018年第1期22-28,共7页
Chinese Journal of Bioinformatics
基金
安徽省生物学重点学科(2014SWQJ009)
关键词
亚洲棉
ARF
进化关系
Gossypium arboretum L.
ARF
Evolution relationship
