摘要
目的:建立同时测定夜寒苏药材挥发油中α-蒎烯、β-蒎烯及芳樟醇含量的方法。方法:按照2015年版《中国药典》(四部)水蒸气蒸馏法进行夜寒苏挥发油的提取。采用气相色谱法测定含量,采用HP-5毛细管色谱柱(30 m×0.032 mm×0.25μm),氢火焰离子化检测器(FID),载气为氮气,进样口温度为200℃,检测器温度为250℃,柱流量为0.8 m L/min,进样量为1μL,分流比为40∶1,程序升温。结果:α-蒎烯、β-蒎烯及芳樟醇的线性范围分别为0.090 5~2.413 3 mg/m L(r=0.999 9)、0.098 3~2.620 0 mg/m L(r=0.999 9)、0.169 1~4.510 0 mg/m L(r=0.999 8),精密度、稳定性(12 h)、重复性试验的RSD≤2.0%(n=6),平均加样回收率分别为99.84%(RSD=0.49%,n=6)、100.24%(RSD=1.38%,n=6)、99.41%(RSD=1.67%,n=6)。23批黔产夜寒苏样品挥发油中α-蒎烯、β-蒎烯及芳樟醇含量分别在0.214 4~1.325 0、0.766 2~3.172 1、0.357 4~1.518 7 mg/g范围内。结论:本试验建立的方法快速、简便、准确,可用于夜寒苏药材挥发油中α-蒎烯、β-蒎烯及芳樟醇含量的测定。
OBJECTIVE:To establish a method for simultaneous determination ofα-pinene,β-pinene and linalool in volatile oil of Hedychium flavum.METHODS:The volatile oil was extracted from H.flavum according to steam distillation stated in Chinese Pharmacopoeia(2015 edition volumeⅣ).GC method was adopted to determine the content.The determination was performed on HP-5 capillary chromatographic column(30 m×0.032 mm×0.25μm)with hydrogen flame ion detector(FID),nitrogen gas as carrier gas,with injector temperature of 200℃,and detector temperature of 250℃,column flow rate of 0.8 mL/min,samples size of 1μL and split ratio of 40∶1 by programmed temperature.RESULTS:The linear range ofα-pinene,β-pinene and linalool were 0.090 5-2.413 3 mg/mL(r=0.999 9),0.098 3-2.620 0 mg/mL(r=0.999 9),0.169 1-4.510 0 mg/mL(r=0.999 8),respectively.RSDs of precision,stability(12 h),reproducibility tests were no more than 2.0%(n=6).The average recoveries were 99.84%(RSD=0.49%,n=6),100.24%(RSD=1.38%,n=6),99.41%(RSD=1.67%,n=6),respectively.The contents ofα-pinene,β-pinene and linalool ranged 0.214 4-1.325 0,0.766 2-3.172 1,0.357 4-1.518 7 mg/g in volatile oil from 23 batches of H.flavum produced in Guizhou province.CONCLUSIONS:The method established in this experiment is rapid,simple and accurate.It can be used for the content determination ofα-pinene,β-pinene and linalool in volatile oil from H.flavum.
作者
张彪
黄芳
周汉华
张浪
董明洪
于泽玥
张蜜
ZHANG Biao;HUANG Fang;ZHOU Hanhua;ZHANG Lang;DONG Minghong;YU Zeyue;ZHANG Mi(College of Pharmacy,Guiyang University of TCM,Guiyang 550025,China;School of Pharmacy,Tongren Polytechnic College,Guizhou Tongren 554300,China)
出处
《中国药房》
CAS
北大核心
2018年第7期933-936,共4页
China Pharmacy
基金
贵州省药监局基金(No.3040640150121)
