摘要
目的:建立止嗽定喘口服液HPLC指纹图谱,为评价其质量提供理论依据。方法:采用Agilent TC-C18色谱柱(4.6mm×150mm,5μm),流动相为乙腈(A)-0.6%磷酸水溶液(B),体积流量1.0ml/min;进样量10μL;柱温30℃;梯度洗脱:0~5min,2%~5%A;5~10min,5%~10%A;10~30min,10%~40%A;30~40min,40%~10%A;40~60min,10%~2%A。结果:建立了止嗽定喘口服液HPLC指纹图谱,在确定的方法下,得到10批银黄清肺胶囊的色谱图,并获得了10批样品的相似度;确定3个已知成分。结论:建立的HPLC指纹图谱方法操作简便,准确稳定,重复性好,为止嗽定喘口服液的质量控制提供理论依据。
Objective:to establish a HPLC fingerprint of cough and asthma oral liquid,and provide a theoretical basis for the evaluation of its quality.Methods:Agilent TC-C18 chromatographic column(4.6mm×150mm,5μm)was used,the mobile phase was acetonitrile(A)-0.6%phosphoric acid aqueous solution(B),and the volume flow rate was l.0ml/min;Injection volume 10μL;Column temperature 30°C;Gradient elution:0～5min,2%～5%A;5～10min,5%～10%A;10～30min,10%～40%A;30～40min,40%～10%A;40～60min,10%～2%A.Results:HPLC fingerprint of stop coughing oral liquid was established.The chromatogram of 10 batches of Yinhuang Lung-clearing Capsules was obtained under the determined method and the similarity of 10 batches of samples was obtained.Three known component are identified.Conclusion:the established HPLC fingerprint method is simple,accurate and stable,reproducible,and provide a theoretical basis for the quality control of cough and asthma oral liquid.
作者
罗琨映
郭江彦
Luo Kunying;Guo Jiangyan(College of chemical engineering,Northwest University for Nationalities,Gansu,730030)
出处
《当代化工研究》
2017年第12期135-136,共2页
Modern Chemical Research
基金
西北民族大学国家级大学生创新创业训练计划资助项目(项目编号:201610742051)
