摘要
目的研究N3F对非小细胞肺癌细胞株的抑制增殖作用,并探讨其诱导癌细胞凋亡的作用及机制.方法采用CCK-8法和平板克隆实验法检测N3F对非小肺癌细胞株SPC-A-1、A549增殖的抑制作用;细胞划痕实验法检测药物对肺癌细胞的迁移抑制;Hoechst 33258染色法与流式细胞仪检测药物诱导肺癌细胞凋亡情况;Western blot法检测N3F对SPC-A-1细胞中HDAC3、Bcl-2和Bax蛋白表达水平的影响.结果与对照组比较,N3F组能显著抑制SPC-A-1、A549细胞的增殖,减少细胞形成的集落数,显著抑制细胞迁移,且呈剂量依赖性.N3F可以诱导肺癌细胞凋亡,下调细胞中HDAC3、Bcl-2蛋白表达,同时上调Bax蛋白的表达,其差异有统计学意义(P<0.05,P<0.01).结论 N3F具有抑制肺癌细胞增殖的作用,其机制可能与下调HDAC3的表达、诱导癌细胞凋亡有关.
Objective To study the inhibitory effect of N3F on cell proliferation and the potential mechanism of induction of cell apoptosis in non-small lung cancer cells.Methods CCK-8 and plate cloning methods were used to detect the inhibitory effect of N3F on the proliferation of non-small lung cancer cell lines SPC-A-1 and A549.Wound scratch assay was used to detect the migration inhibition of drugs on lung cancer cells.Hoechst 33258 staining and flow cytometry were used to detect drug-induced apoptosis of lung cancer cells.The expressions of HDAC3 and apoptosis-related proteins Bcl-2 and Bax were detected by western blot.Results Compared with the control group,N3F group significantly inhibited the proliferation of SPC-A-1 and A549 cells,reduced a decrease in colony formation and significantly inhibited cell migration in a dose-dependent manner.N3F also induced lung cancer cell apoptosis,down-regulated HDAC3 and Bcl-2 expression and up-regulated Bax expression(P<0.05,P<0.01).Conclusion N3F can inhibit the proliferation of lung cancer cells,which may be related to down-regulation of HDAC3 expression and induction of cell apoptosis.
作者
倪安然
黄伟斌
王晓翔
孙心愿
冯冰虹
NI Anran;HUANG Weibin;WANG Xiaoxiang;SUN Xinyuan;FENG Binghong(College of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;Department of Clinical Pharmacy,Puning People′s Hospital,Puning 515300,China;College of Pharmacy,Central South University,Changsha 410013,China)
出处
《广东药科大学学报》
CAS
2018年第2期244-248,共5页
Journal of Guangdong Pharmaceutical University
基金
广东省科技计划项目(2013B021800079)
