摘要
目的:在ISSR分子标记方法的基础上找到属于正毛化橘红的PCR特征条带或特征条带组合来建立鉴别正毛化橘红种苗的方法。方法:在50条ISSR引物中筛选合适的引物对三个品种的化橘红叶片样品进行PCR扩增及电泳条带分析,寻找正毛化橘红的特征条带或条带组合,并对80份化橘红叶片样品进行验证。结果:寻找到了两个引物的特征条带组合可以用来鉴别正毛化橘红种苗,建立了正毛化橘红种苗分子鉴定方法。结论:本文建立的基于ISSR分子标记的PCR特征条带组合的方法可以用来快速准确的鉴别正毛化橘红种苗。
Objective:The study aims at establishing an identification method of Zhengmao Exocarpium Citri Grandis by seeking out the characteristic PCR band or bands combination belonging to Zhengmao Citri Grandis Exocarpium based on ISSR molecular markers.Methods:Proper primers were screened out for PCR,electrophoresis and cluster analysis within 50 ISSR primers.The characteristic PCR band or bands combination belonging to Zhengmao Citri Grandis Exocarpium were sought out and 80 leaf samples of Citri Grandis Exocarpium were validated.Results:The characteristic PCR bands combination of two primers were sought out to identify Zhengmao Citri Grandis Exocarpium and the molecular identification method was established.Conclusion:The established method of characteristic PCR bands combination based on ISSR molecular markers can be used to identify Zhengmao Citri Grandis Exocarpium quickly and accurately.
作者
胡珊
杨志业
邬龙怡
李华
计周正
HU Shan;YANG Zhi-ye;WU Long-yi;LI Hua;JI Zhou-zheng(Guangzhou Xiangxue Pharmaceutical Limited by Share Ltd,Guangzhou 510663,China;Guangdong institute.for Drug Control,Guangzhou 510180,China)
出处
《中国现代中药》
CAS
2018年第6期663-668,共6页
Modern Chinese Medicine
基金
广东省中医药局科研项目(20171041)
关键词
ISSR分子标记
正毛化橘红
特征条带
ISSR Molecular Markers
Zhengmao Citri Grandis Exocarpium
characteristic bands
