摘要
首先用十二烷基硫酸钠(SDS)将壳聚糖(CS)的氨基进行保护,得到油溶性产物SCS;以二丁基镁为引发剂引发β-丁内酯开环聚合合成聚β-羟基丁酸酯(PHB),以六亚甲基二异氰酸酯(HDI)为改性剂对PHB端基进行活化;然后将PHB接枝到SCS分子链上得到两亲性接枝聚合物SCS-g-PHB;再将壳聚糖氨基脱保护,得到CS-g-PHB;用丁二酸酐酰化得到具有一定pH值响应性的丁二酸酐酰化接枝聚合物SC-g-PHB;最后,通过层层自组装技术制备了具有核壳结构的SC-g-PHB纳米微球;并通过FTIR、1 HNMR、TGA、SEM、TEM和DLS对其结构进行了表征。结果表明,CSg-PHB的接枝率为21.5%,SC-g-PHB纳米微球具有明显的核壳结构,粒径在200nm左右,是一种新型的靶向药物载体。
Firstly,we protected the amino group on chitosan(CS)with sodium dodecyl sulfate(SDS),and obtained oil-soluble product SCS.We synthesized PHB via ring-opening polymerization ofβ-butyrolactone with dibutylmagnesium as an initiator,and activated the terminal group of PHB using hexamethylene diisocyanate(HDI)as a modifier.Then,we grafted PHB onto SCS to obtain amphipathic grafted polymer SCS-g-PHB,obtained CS-g-PHB through amino deprotection of CS,and got succinic anhydride acylated grafted polymer SC-g-PHB with a certain pH value responsibility by the acylation of succinic anhydride.Finally,we prepared SC-g-PHB nanosphere with a core-shell structure by layer-by-layer self-assembly technique.Moreover,we characterized the structure of the product by FTIR,1HNMR,TGA,SEM,TEM,and DLS.The results show that the graft rate of CS-g-PHB is 21.5%,SC-g-PHB nanosphere exhibits an obvious core-shell structure with the particle size of about 200 nm,which is a novel targeting drug carrier.
作者
王先津
代元坤
苑志磊
贺继东
WANG Xian-jin;DAI Yuan-kun;YUAN Zhi-lei;HE Ji-dong(Key Laboratory of Rubber-Plastics of Ministry of Education,Qingdao University of Science&Technology,Shandong Provincial Key Laboratory of Rubber-Plastics,Qingdao 266042,China)
出处
《化学与生物工程》
CAS
2018年第7期42-46,共5页
Chemistry & Bioengineering
关键词
壳聚糖
聚Β-羟基丁酸酯
丁二酸酐
酰化
两亲性接枝聚合物
层层自组装
纳米微球
chitosan
polyβ-hydroxybutyrate
succinic anhydride
acylation
amphiphilic grafted polymer
layer-by-layer self-assembly
nanosphere
