摘要
【目的】建立液相色谱-串联质谱法同时测定蛋黄中4种类胡萝卜素着色剂(角黄素、柑橘皮类胡萝卜素、β-阿朴-8'-胡萝卜素酸乙酯和β-阿朴-8'-胡萝卜素醛)的方法。【方法】蛋黄经乙腈提取,采用Waters Xterra MS C18色谱柱分离;质谱采用电喷雾正离子电离模式和多反应扫描监测模式进行测定,以外标法定量。【结果】4种类胡萝卜素着色剂的检出限均为2.5 ng/g;在2.5~200ng/g范围内线性关系良好,相关系数r>0.99;回收率在82.24%~109.2%之间,相对标准偏差为1.0%~3.0%(n=6)。【结论】本方法灵敏度高、准确性好,适用于蛋黄中4种类胡萝卜素着色剂的检测。
[Objective]To establish a method for simultaneous determination of four carotenoid pigments(canzthaxanthin,citranaxanthin,β-apo-8-carotenoic acid ethyl ester,β-apo-8-carotenal)in yolk by liquid chromatography-tandem mass spectrometry(LC/MS/MS).[Methods]Yolk was extracted by acetonitrile,and then separated by Waters Xterra MS C18 column.Mass spectrometry used electrospray ionization positive ionization mode(ESI+)and multiple reaction monitoring(MRM)for determination with external standard method for quantification.[Results]The detection limit of the 4 carotenoid pigments were 2.5 ng/g.The method showed a good linearity over the range of 2.5~200 ng/g with r>0.99.The average recoveries were in the range of 82.24%~109.2%,while the relative standard deviations(RSD)were 1.0%~3.0%(n=6).[Conclusion]The established method is highly sensitive and accurate,which is suitable for the detection of four carotenoid pigments in yolk.
作者
焦广睿
王柯
JIAO Guang-rui;WANG Ke(Food and Cosmetics Department,Shanghai Institute of Food and Drug Control,Shanghai 201203,China)
出处
《上海预防医学》
CAS
2018年第6期472-475,共4页
Shanghai Journal of Preventive Medicine
