摘要
[目的]本试验旨在探讨AMP激活蛋白激酶α1和α2基因(PRKAA1、PRKAA2)在高、低饲料效率组肉鸭胸大肌、腿肌和肝脏中表达水平及其与剩余采食量(RFI)性状的相关性。[方法]选取体质量相近的H系肉公鸭1 000只,测定21~42日龄时的采食量和体增重,计算RFI。挑选高、低RFI组肉鸭各8只,采用定量PCR检测PRKAA1和PRKAA2在2组肉鸭胸大肌、腿肌和肝脏中表达水平。[结果]PRKAA1 mRNA在低RFI组肉鸭胸大肌中表达量极显著高于高RFI组(P<0.01),腿肌中表达量显著高于高RFI组(P<0.05)。低RFI组胸大肌和腿肌中PRKAA2 mRNA表达量显著高于高RFI组(P<0.05)。相关性分析表明,肉鸭胸大肌中PRKAA1 mRNA相对表达量与日采食量、饲料转化率和RFI显著负相关(P<0.05),腿肌中相对表达量与日采食量和RFI显著负相关(P<0.05)。另外,胸大肌中PRKAA2 mRNA相对表达量与日采食量和RFI显著负相关(P<0.05),腿肌中相对表达量与RFI呈显著负相关关系(P<0.05)。[结论]PRKAA1和PRKAA2基因在高、低RFI组肉鸭胸大肌和腿肌中表达量存在显著差异且与剩余采食量性状显著相关。
[Objectives]The objective of this study was to examine expression levels of AMP-activated protein kinase catalytic subunit alpha-1(PRKAA 1)and alpha-2(PRKAA 2)mRNA in pectoralis major muscle,leg muscle and liver and their correlations with residual feed intake in meat-type ducks with high and low residual feed intake.[Methods]A total of 1 000 male H-line ducks with similar body weight were chosen to record feed intake and body weight gain from 21 to 42 day old,then calculated residual feed intake(RFI)in the interval.At 42 d,the 8 high RFI(HRFI)and 8 low RFI(LRFI)ranking ducks were selected to detect relative expressions of PRKAA 1 and PRKAA 2 mRNA in pectoralis major,leg muscle and liver in meat-type ducks between HRFI and LRFI group.[Results]The results showed that expression level of PRKAA 1 mRNA in pectoralis major of LRFI group was significantly higher than that of HRFI group(P<0.01),and its relative expression in leg muscle of LRFI group was significantly higher than that of HRFI group(P<0.05).Relative expression of PRKAA 2 mRNA in pectoralis major and leg muscle of LRFI group was significantly greater than that of HRFI group(P<0.05).The correlation analysis demonstrated that relative expression of PRKAA 1 mRNA in pectoralis major was significantly negatively correlated with average daily feed intake(ADFI),feed conversion ratio(FCR)and RFI(P<0.05),and its expression level in leg muscle was negatively associated with ADFI and RFI(P<0.05).Additionally,expression level of PRKAA 2 mRNA in pectoralis major was significantly negatively correlated with ADFI and RFI(P<0.05),and its relative expression in leg muscle was significantly negatively related to RFI(P<0.05).[Conclusions]The results of this study indicate that the expression levels of PRKAA 1 and PRKAA 2 in pectoralis major and leg muscle between HRFI and LRFI group are statistically significant and their relative expressions are significantly associated with residual feed intake in meat-type ducks.
作者
金四华
杨磊
童雨翠
范心凤
何婷婷
汪加发
李永胜
耿照玉
JIN Sihua;YANG Lei;TONG Yucui;FAN Xinfeng;HE Tingting;WANG Jiafa;LI Yongsheng;GENG Zhaoyu(College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China;Huangshan Qiangying Duck Breeding Co.,Ltd.,Huangshan 245461,China;Extension Center for Animal Husbandry and Veterinary Medicine of Huangshan City,Huangshan 245000,China)
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2018年第5期925-930,共6页
Journal of Nanjing Agricultural University
基金
安徽省重点研究与开发计划项目(1704a07020091)
安徽省自然科学基金项目(1808085QC61)
地方畜禽遗传资源保护与生物育种省重点实验室项目(AKLGRCB2017008)
安徽农业大学引进人才项目(yj2017-03)
关键词
鸭
剩余采食量
PRKAA1
PRKAA2
相关性
duck
residual feed intake
AMP-activated protein kinase catalytic subunit alpha-1(PRKAA 1)
AMP-activated protein kinase catalytic subunit alpha-2(PRKAA 2)
correlation
