摘要
背景:富白细胞-血小板血浆和富白细胞-血小板纤维蛋白是两种具有加快创面愈合功效的自体富血小板凝胶。目的:比较富白细胞-血小板纤维蛋白及富白细胞-血小板血浆在组成成分、结构、释放生长因子方面的差异?方法:抽取志愿者空腹静脉血,每份血样分别制备富白细胞-血小板纤维蛋白凝胶与富白细胞-血小板血浆凝胶,采用光镜及扫描电镜观察凝胶的微观结构,计算白细胞、血小板的浓缩倍数及回收率。将两种凝胶保存于DMEM培养基中,1,3,7,14,21 d后收集含有渗出液的培养液,ELISA法检测血小板源性生长因子、转化生长因子β1、血管内皮细胞生长因子的浓度。结果与结论:(1)光镜下显示,富白细胞-血小板纤维蛋白凝胶内的血小板?白细胞与红细胞分界清晰,可见大量白细胞及纤维蛋白簇集;富白细胞-血小板血浆凝胶的血小板、白细胞较少,散在红细胞中;(2)电镜下显示,富白细胞-血小板纤维蛋白凝胶中含有大量纤维蛋白,形成致密的特殊3D结构,交界处血小板叠加结构清晰;富白细胞-血小板血浆凝胶交界处纤维较少且夹杂大量血小板,部分血小板已裂解;(3)与富白细胞-血小板血浆凝胶比较,富白细胞-血小板纤维蛋白凝胶具有更高的血小板回收率(P=0)、白细胞回收率(P=0)及淋巴细胞回收率(P=0);(4)除血管内皮细胞生长因子外,富白细胞-血小板纤维蛋白凝胶保存3,7,14,21 d的转化生长因子β1、血小板源性生长因子浓度高于富白细胞-血小板血浆凝胶;(5)结果表明与富白细胞-血小板血浆凝胶相比,富白细胞-血小板纤维蛋白凝胶制备更简单、结构更优、生长因子释放更缓慢,在治疗创面中更有优势。
BACKGROUND:Leukocyte-and platelet-rich plasma(L-PRP)and leukocyte-and platelet-rich fibrin(L-PRF)are two kinds of autologous platelet rich gels that can accelerate wound healing.OBJECTIVE:To compare the differences in the composition,structure and release of growth factors between L-PRP and L-PRF.METHODS:Fasting blood samples were collected from healthy volunteers to prepare L-PRP and L-PRF gels.The microstructure of the gels was observed by light microscope and scanning electron microscope,and the concentration times and recovery rates of leukocytes and platelets were calculated.The two gels were stored in DMEM medium.After 1,3,7,14 and 21 days,the culture fluid containing exudate was collected.The platelet-derived growth factor,transforming growth factorβ1,and vascular endothelial growth factor were detected by ELISA.RESULTS AND CONCLUSION:(1)Under the light microscope,platelet,leukocytes and erythrocytes were clearly demarcated,and leukocytes and fibrin aggregated in cluster.The platelets and leukocytes in the L-PRP gel were less in number and dispersed among erythrocytes.(2)Under the electron microscope,a large amount of fibrins were in the L-PRF gel,to form a dense special 3D structure,and the platelet superposition structure at the junction was clear.There were less fibers and a large number of platelets at the junction of L-PRP gel,and some of the platelets were cleaved.(3)Compared with the L-PRP gel,the L-PRF had a higher platelet recovery rate(P=0),leukocyte recovery rate(P=0)and lymphocyte recovery rate(P=0).(4)In addition to the vascular endothelial cell growth factor,the levels of transforming growth factorβ1 and platelet-derived growth factor in the L-PRF gel stored for 3,7,14 and 21 days were higher than those in the L-PRP gel.These findings indicate that compared with the L-PRP gel,the L-PRF gel is easier to prepare,has better structure and slower release of growth factors,which have more advantages in the treatment of wounds.
作者
武钰翔
司少艳
许樟荣
王爱红
Wu Yuxiang;Si Shaoyan;Xu Zhangrong;Wang Aihong(Department of Endocrinology,the 306th Hospital of PLA,Clinical Medicine College of Anhui Medical University,Beijing 100101,China;Special Medical Laboratory and Research Center,Beijing 100101,China;Department of Endocrinology,the 306th Hospital of PLA,Beijing 100101,China)
出处
《中国组织工程研究》
CAS
北大核心
2018年第34期5484-5489,共6页
Chinese Journal of Tissue Engineering Research
基金
全军医学科技青年培育计划拔尖项目(16QNP041)
首都临床特色应用研究(Z141107002514181)
中华医学会临床医学科研专项资金项目(12020580358)。
