摘要
目的探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)程序性死亡分子-1(programmed death-1,PD-1)/程序性死亡分子配体-1(programmed death ligand 1,PD-L1) mRNA与EGFR基因突变的相关性。方法采用RNAscope法检测PD-1、PD-L1 mRNA的表达,应用基因测序法检测EGFR基因的突变类型。结果 64例NSCLC患者中,PD-1、PD-L1 mRNA的阳性率分别为40. 6%和35. 9%。PD-1、PD-L1 mRNA的表达与患者性别、吸烟史、分化程度、淋巴结转移及TNM分期均无关(P>0. 05)。PD-L1 mRNA的表达与患者年龄相关,60岁以上患者PD-L1 mRNA阳性率较高(P <0. 05)。64例NSCLC样本中发生EGFR基因突变27例(占42. 2%),EGFR基因突变与患者性别、吸烟史、组织学类型及分化程度相关(P <0. 05)。EGFR基因突变率在女性、无吸烟史、腺癌及高分化患者中突变率高。相关分析显示,PD-L1 mRNA表达与EGFR基因突变呈正相关(r=0. 283,P <0. 05)。结论 NSCLC患者PD-L1 mRNA表达与EGFR基因突变呈正相关。
Purpose To investigate the correlation of PD-1,PD-L1 mRNA expression and EGFR gene mutation in non-small cell lung cancer(NSCLC).Methods The mRNA of PD-1 and PD-L1 was detected by RNAscope,and the EGFR mutation was detected by gene sequence.Results The positive rate of PD-1 and PD-L1 mRNAs was 40.6%and 35.9%respectively in NSCLC.There was no relationship between PD-1 and PD-L1 mRNA expression with the gender,smoking condition,differentiation,lymph node metastasis and TNM stages(P>0.05).The expression of PD-L1 mRNA was related to patient’s age in NSCLC(P<0.05).PD-L1 mRNA expression rate was higher in over 60 years of age patients.There was 27 patients with EGFR gene mutation in 64 patients with NSCLC.The mutation rate of the EGFR gene was 42.2%.The mutation rate of EGFR was correlated with patients’sex,smoking history,histological type and differentiation in 64 cases of NSCLC(P<0.05).The mutation was higher in female,no smoking,and well-differentiated adenocarcinoma patients.The correction analysis showed that there was positive relationship between PD-L1 mRNA expression with EGFR mutation(r=0.283,P<0.05).Conclusion There is positive relationship between PD-L1 mRNA expression with EGFR mutation.
作者
王珊
董丽儒
任会强
宋旭东
WANG Shan;DONG Li-ru;REN Hui-qiang;SONG Xu-dong(Department of Pathology,the Affiliated Hospital,North China University of Science and Technology,Tangshan 063000,China;Department of Pathology,the First People’s Hospital of Jinzhong,Jinzhong,Shanxi Province 030600,China)
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2018年第10期1091-1095,共5页
Chinese Journal of Clinical and Experimental Pathology
基金
河北省政府临床医学优秀人才培养和基础课题研究(361036)
