摘要
针对纺织服装中双酚A(BPA)分子量小,直接采用表面等离子体共振(SPR)技术难以精准检测的问题,采用传感器表面预处理方法和溶液竞争原理来扩大响应信号,对其进行定性定量检测。为更好地使双酚A-牛血清白蛋白抗原(BPA-BSA)和传感芯片进行氨基偶联,从传感芯片的选择、修饰溶液的pH值、离子浓度、BPA-BSA抗原的最佳浓度4个因素出发,运用控制变量法,探索双酚A检测中传感芯片的最佳修饰条件。结果表明:当采用羧甲基葡聚糖组装(CM5)芯片、修饰液pH值为4.5、离子浓度为10 mmol/L、BPA-BSA质量浓度为50μg/mL时,BPA-BSA的氨基偶联修饰效果最佳,且预处理方法简便快捷,BPA无需精度提纯,特异性识别度高,可应用在纺织品双酚A检测中。
The bisphend A in textiles and clothing has low molecular weight,that is difficult to be accurately measured.The surface plasmon resonance(SPR)and solution competition method was used to detect it.In order to better bind bisphenol A and bovine serum albumin antigens(BPA-BSA)with sensing chips to generate amino groups,four factors including selection of the sensing chip,the modification of pH for the solutions,the ion concentration and the optimal concentration of BPA-BSA were taken into account.By controlling the variates of the four factors,the best condition for sensor chip to detect BPA was determined.The results show that the best amino-coupled effect of BPA-BSA occurs using carboxymethyl dextran assembly(CM5)chip at pH 4.5,the ion concentration of 10 mmol/L and the concentration of BPA-BSA of 50μg/mL.The pretreatment method is simple and efficient,and high specificity BPA an detected without precision purification.This method can be used in textiles for BPA detection.
作者
李正海
刘芳
黄珏
薛文良
钱竞芳
LI Zhenghai;LIU Fang;HUANG Jue;XUE Wenliang;QIAN Jingfang(Key Laboratory of Textile Science&Technology,Ministry of Education,Donghua University,Shanghai 201620,China;CNTAC Testing Service Co.,Ltd.(Foshan),Foshan,Guangdong 528000,China;Shanghai Customs District People’s Republic of China,Shanghai 200120,China)
出处
《纺织学报》
EI
CAS
CSCD
北大核心
2018年第11期145-149,共5页
Journal of Textile Research
基金
海关总署科研计划资助项目(2018IK015)
上海市技术标准资助项目(18DZ2205900)
关键词
纺织品
双酚A
表面等离子体共振
氨基偶联
溶液竞争法
textile
bisphenol A
surface plasmon resonance
amino coupling
solution competition method
