摘要
背景:研究表明小鼠体内过氧化物酶体增殖活化受体γ辅助活化因子1α(peroxisome proliferators-activated receptor-γcoactivator-1α,PGC-1α)蛋白、Ⅲ型纤维连接蛋白域蛋白5(fibronectin typeⅢdomain-containing protein 5,FNDC5)蛋白和Irisin组成一条正向调节脂肪分解代谢的通路,并且PGC-1α能调控骨骼肌中调节脂肪代谢相关蛋白PPARδ的表达。耐力运动训练可以有效减轻小鼠体质量,但不同强度耐力运动训练对高脂诱导的肥胖小鼠血清Irisin含量及骨骼肌中PGC-1α、FNDC5、PPARδ蛋白表达的影响尚不清楚。目的:分析不同强度耐力运动训练对高脂诱导肥胖小鼠血液中Irisin含量及骨骼肌中脂肪代谢相关蛋白的影响。方法:4周龄雄性C57BL/6J小鼠经过1周常规饲料适应性喂养后,通过8周高脂饲料喂养建立营养性肥胖小鼠模型,对照小鼠一直使用常规饲料喂养。构建成功的肥胖小鼠经过1周适应性跑台运动后,分为高强度耐力运动组和低强度耐力运动组进行跑台运动,对照小鼠不运动。8周后量小鼠体长、称量体质量并计算Lee’s指数,同时取各组小鼠血清和股四头肌,使用ELISA方法检测小鼠血清中Irisin水平并对比各组变化,使用Western Blot方法检测小鼠股四头肌中PGC-1α、FNDC5和PPARδ蛋白的表达情况并对比各组变化。结果与结论:①高脂诱导能够明显增大小鼠的体长、体质量和Lee’s指数,表明高脂诱导的营养性肥胖小鼠模型是成功的,并且高脂诱导能明显降低小鼠血清Irisin水平,降低小鼠股四头肌脂质代谢相关蛋白PGC-1α、FNDC5和PPARδ的表达;②耐力运动训练尤其是高强度耐力运动训练能有效减小肥胖小鼠体长、体质量和Lee’s指数;③耐力运动训练尤其是高强度耐力运动训练能有效提高肥胖小鼠血清中Irisin水平,明显提高小鼠股四头肌PGC-1α、FNDC5和PPARδ蛋白的表达;④ PGC-1α和FNDC5蛋白与Irisin的变化一致,提示耐力运动训练尤其是高强度耐力运动训练可以诱导骨骼肌高表达PGC-1α蛋白,从而促进FNDC5蛋白高表达,随着FNDC5蛋白在体内被剪切转变成高浓度的Irisin释放到血液中,加速小鼠脂肪代谢水平,达到减肥的目的。
BACKGROUND:Peroxisome proliferators-activated receptor-γcoactivator-1 alpha(PGC-1α),fibronectin type III domain-containing protein 5(FNDC5)and Irisin in mice have been shown to make a positive pathway of lipolysis,and PGC-1αcan regulate the expression of peroxisome proliferator-activated receptor delta(PPARδ),which regulates lipolysis in skeletal muscle.Endurance training can effectively reduce the body mass of mice,but the effect of different intensities of endurance training on serum Irisin level,and PGC-1α,FNDC5,PPARδexpression in mice with obesity induced by a high fat diet is still unclear.OBJECTIVE:To study the effect of high-and low-intensity endurance training on serum Irisin level,and lipolysis-related proteins in mice with obesity induced by a high fat diet.METHODS:Four-week-old male C57BL/6J mice were fed normal diet for 1 week and then fed 8 weeks of high-fat diet to establish a mouse model of nutritional obesity.The control mice were fed a normal diet.After 1 week of adaptive treadmill training,the obese mice were divided into high-and low-intensity endurance training groups for treadmill training and the control group had no training.After 8 weeks,the body length,body mass and Lee’s index were measured and recorded.The level of Irisin in mouse serum was detected by ELISA.The expression levels of PGC-1α,FNDC5 and PPARδin quadriceps femoris were detected by western blot assay.RESULTS AND CONCLUSION:High-fat diet could significantly increase body length,body mass and Lee’s index of mice,suggesting that high-fat-induced nutritional obesity mouse model was established.High-fat diet could significantly reduce the level of Irisin in serum and the expression of lipolysis-related proteins PGC-1α,FNDC5 and PPARδin quadriceps femoris.Endurance training,especially high-intensity endurance training could effectively reduce the body length,body mass and Lee’s index of mice.Endurance training,especially high-intensity endurance training could effectively increase the level of Irisin in serum and the expression of PGC-1α,FNDC5 and PPARδin quadriceps femoris.PGC-1α,FNDC5 and Irisin have consistent expression,suggesting that endurance training,especially high-intensity endurance training can up-regulate the expression of skeletal muscle PGC-1α,thereby increasing the expression of FNDC5 protein.FNDC5 is transformed into Irisin and released into the blood,thus increasing the level of lipolysis in mice,and achieving weight loss.
作者
苏坤霞
Su Kunxia(Public Education Department of Xin Lian College,Henan Normal University,Zhengzhou 451400,Henan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第3期427-434,共8页
Chinese Journal of Tissue Engineering Research
