摘要
为揭示应激导致家禽肝脏代谢异常的机理,本研究探查了糖皮质激素——地塞米松(DEX)对鸡胚肝细胞脂肪和胆汁酸代谢相关基因表达的影响。选取19胚龄的无特定病原体(SPF)鸡蛋,原代培养鸡胚肝细胞(37℃、5%CO2),用0(对照)、200、500、1 000 nmol/L的地塞米松分别处理24 h。结果表明:与对照组相比,高剂量(500、1 000 nmol/L)地塞米松处理显著降低了脂肪代谢相关基因——脂肪酸转运蛋白1 (FATP-1)、固醇调节元件结合蛋白-1C(SREBP-1C)、载脂蛋白B100(APOB100)、肝脏X受体(LXR)以及胆汁酸摄取相关基因Na+/牛磺胆盐共转运体(NTCP)的mRNA相对表达水平(P<0.05),显著提高了胆汁酸排出相关基因——胆盐输出泵(BSEP)的mRNA相对表达水平(P<0.05);低剂量(200 nmol/L)地塞米松处理显著提高胆汁酸合成相关基因——胆固醇7-羟化酶(CYP7A1)和法尼酯X受体(FXR)的mRNA相对表达水平(P<0.05),同时,SREBP-1C的mRNA相对表达水平显著降低(P <0.05),BSEP的mRNA相对表达水平显著上升(P<0.05)。由此得出,高剂量糖皮质激素对鸡胚肝细胞的脂肪合成、转运和胆汁酸的摄取具有抑制作用;低剂量糖皮质激素可以促进胆汁酸的合成和排出,部分反应具有剂量依赖性。
In order to reveal the mechanism of stress-induced metabolism disorders of liver in poultry,this study explored the effect of glucocorticoid-dexamethasone(DEX)on the expression of fat and bile acid metabolism related genes in chicken embryo hepatocytes.Nineteen embryo age specific pathogen free(SPF)eggs were selected,and primary cultured(37℃,5%CO 2)chicken embryo hepatocytes were treated with 0(control),200,500,and 1 000 nmol/L dexamethasone respectively for 24 h.The results showed as follows:compared with the control group,treated with the high dose(500 or 1 000 nmol/L)dexamethasone significantly reduced the mRNA relative expression levels of fat metabolism related genes,such as fatty acid transporter 1(FATP-1),sterol regulatory element binding protein-1C(SREBP-1 C),apolipoprotein B100(APOB100)and liver X receptor(LXR)(P<0.05),and the mRNA relative expression level of bile acid uptake related gene Na+/taurocholate co-transporting polypeptide(NTCP)was also reduced(P<0.05);While the mRNA relative expression level of bile acid excretion related gene bile salt output pump(BSEP)was significantly increased(P<0.05);treated with the low dose(200 nmol/L)dexamethasone significantly increased the mRNA relative expression levels of bile acid synthesis related genes cholesterol7-alpha hydroxylase(CYP7A1)and farnesoid X receptor(FXR)(P<0.05),meanwhile,the SREBP-1 C mRNA relative expression level was significantly decreased(P<0.05),and the BSEP mRNA relative expression level was significantly increased(P<0.05).In conclusion,high dose glucocorticoids can inhibit the synthesis and transport of fat and bile acid intake in chicken embryo hepatocytes,while low dose glucocorticoids can promote the synthesis and excretion of bile acids,some of these reactions have a dose-dependent response.
作者
周华金
杨家昶
张辉
王继光
郝洋洋
杜鹏
宋志刚
ZHOU Huajin;YANG Jiachang;ZHANG Hui;WANG Jiguang;HAO Yangyang;DU Peng;SONG Zhigang(College of Animal Science and College of Veterinary Medicine,Shandong Agricultural University,Tai’an 271018,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2018年第10期4262-4268,共7页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金项目(31472115)
山东省家禽产业创新团队项目(STAIT-011-08)
关键词
糖皮质激素
鸡胚肝细胞
脂肪代谢
胆汁酸合成
基因表达
glucocorticoids
chicken embryos hepatocyte
fat metabolism
bile acid synthesis
gene expression
