摘要
为缓解环磷酸腺苷(cyclic adenosine monophosphate,cAMP)发酵能量供应不足的问题,发酵24 h一次性添加3 g/L-broth的柠檬酸钠,cAMP产量达到3. 69 g/L,发酵强度为0. 074 g/(L·h),分别比对照提高了11. 2%和60. 2%,发酵性能得到改善。通过对胞内能量物质含量进行比较,发现添加柠檬酸钠显著提高了ATP含量和ATP/ADP比率,为cAMP合成提供了能量和物质保障。另外,提出了添加柠檬酸钠偶合脉冲补充次黄嘌呤(前体)的发酵工艺,将能量供给与补救途径的优势相结合,cAMP产量达到4. 42 g/L,发酵强度为0. 088g/(L·h),分别比对照提高了33. 1%和91. 3%。
As an auxiliary energy substrate,citrate could enhance the synthesis of ATP and most of the high energy-consuming products.3 g/L-broth citrate was added into the broth for one time at 24 h for energy supply to produce 3.69 g/L cAMP with the productivity of 0.074 g/(L·h).The performance was improved greatly with cAMP concentration and productivity enhancement of 11.2%and 60.2%,respectively,when compared with control.The results of energy substrate analysis indicated that ATP amounts and ATP/ADP ratios were enhanced notable by sodium citrate.In addition,a cAMP fermentation process with sodium citrate coupling hypoxanthine addition in pulses was proposed and cAMP concentration achieved 4.42 g/L with a productivity of 0.088 g/(L·h),which was improved 33.1%and 91.3%,respectively due to the integrated function of energy supply and salvage pathway.
作者
李志刚
陈宝峰
方智博
张中华
常景玲
LI Zhi-gang;CHEN Bao-feng;FANG Zhi-bo;ZHANG Zhong-hua;CHANG Jing-ling(Collaborative Innovation Center of Modern Biological Breeding of Henan Province,Xinxiang 453003,China;School of Life Science and Technology,Henan Institute of Science and Technology,Xinxiang 453003,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2018年第11期154-158,共5页
Food and Fermentation Industries
基金
河南省科技攻关项目(社会发展)(162102310419)
关键词
柠檬酸盐
环磷酸腺苷
次黄嘌呤
补救途径
citrate
cyclic adenosine monophosphate
hypoxanthine
salvage pathway
