摘要
目的优化地黄多糖超声提取工艺,并评价其抗氧化活性。方法在单因素试验基础上,以料液比、提取时间、提取温度、醇沉浓度为影响因素,多糖得率为评价指标,正交试验优化超声提取工艺。然后,考察分步醇沉(50%、70%、80%、90%乙醇,相应部位分别命名为RGPS50、RGPS70、RGPS80、RGPS90)对6个品种中多糖含有量的影响,以及多糖对DPPH自由基的清除作用。结果最佳条件为料液比1∶30,提取时间100 min,提取温度50℃,醇沉浓度90%,多糖得率7. 75%。各品种中多糖含有量依次为85-5>星科>金九>沁怀>北京3号>怀丰。RGPS90中多糖含有量最高,抗氧化活性最弱; RGPS80中多糖含有量相对较低,抗氧化活性最强。结论该方法稳定可靠,可用于超声提取地黄多糖。不同品种、醇沉部位地黄中多糖含有量和抗氧化活性差异明显。
AIM To optimize the ultrasonic extraction process for polysaccharides from Rehmannia glutinosa Libosch.,and to evaluate the antioxidant activity. METHODS With solid-liquid ratio,extraction time,extraction temperature,ethanol precipitation concentration as influencing factors,polysaccharides yield as an evaluation index,orthogonal test was applied to optimizing the ultrasonic extraction process on the basis of single factor test.Subsequently the effects of stepwise ethanol precipitation(ethanol at concentrations of 50%,70%,80%,90%,whose corresponding fractions were named as RGPS50,RGPS70,RGPS80,RGPS90,respectively) on the contents of six polysaccharides were investigated,then the scavenging effect of polysaccharides on DPPH free radical was detected. RESULTS The optimal conditions were determined to be 1 ∶ 30 for solid-liquid ratio,100 min for extraction time,50 ℃ for extraction temperature,and 90% for ethanol precipitation concentration,the polysaccharides yield was 7. 75%. The contents of various polysaccharides were found in sequence of 85-5 > Xingke > Jinjiu > Qinhuai > Beijing No. 3 > Huaifeng. Yet the polysaccharides in RGPS90,with the highest content,displayed the weakest antioxidant activity; while the polysaccharides in RGPS80,with relatively low content,showed the strongest antioxidant activity. CONCLUSION This stable and reliable method can be used for the ultrasonic extraction for polysaccharides from R. glutinosa. Different varieties and ethanol precipitation fractions of R. glutinosa polysaccharides demonstrate obvious differences in contents and antioxidant activities.
作者
张骆琪
刘苏伟
王飞
刘玉霞
鲁传涛
刘红彦
ZHANGLuo-qi;LIU Su-wei;WANG Fei;LIU Yu-xia;LU Chuan-tao;LIU Hong-yan(Henan University of Chinese Medicine,Zhengzhou 450046,China;Institute for Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)
出处
《中成药》
CAS
CSCD
北大核心
2018年第12期2662-2667,共6页
Chinese Traditional Patent Medicine
基金
国家中药材产业技术体系郑州综合实验站(CARS-21-12)
河南省科技攻关项目(172102310191)
关键词
地黄
多糖
超声提取
抗氧化活性
正交试验
Rehmannia glutinosa Libosch.
polysaccharides
ultrasonic extraction
antioxidant activity
orthogonal test
