摘要
研究生、制首乌正己烷提取物对维甲酸致小鼠骨质疏松的防治作用,并探讨其主要作用机制。采用维甲酸(105 mg·kg-1)致小鼠骨质疏松模型,生首乌正己烷提取物高、低剂量组48、24 mg·kg-1,清蒸制首乌正己烷提取物高、低剂量组48、24 mg·kg-1,黑豆制何首乌正己烷提取物高、低剂量组48、24 mg·kg-1,灌胃给药,2周后以比色法测定血清钙、磷含量和碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)活性,竞争放射免疫法测定血清中骨钙素(BGP)和雌二醇(E2)含量,取股骨进行骨组织形态学观察。结果表明:维甲酸可引起高转换型骨质疏松,与模型组比较,生、制首乌各剂量组可显著升高s-E2的水平(P <0. 05),降低s-ALP、s-TRAP和s-BGP的水平(P <0. 05,P <0. 01);生、制首乌各组与模型组比较,骨组织形态均有不同程度的改善,骨小梁数明显增加。从以上结果可知,生、制首乌正己烷提取物对维甲酸致小鼠骨质疏松有明显的治疗作用,其机制可能与减缓雌激素水平降低,抑制骨高转换有关。
We investigated the anti-osteoporosis effect and the possible mechanism of PM-HE(Hexane extract from Raw and Processed Polygonum multiflorum)in animal model.Kunming mice were randomly divided into9groups:Control group(normal saline),model group,nilestriol group(2mg/kg),PM-HE groups(48,24mg/kg).The mice osteoporosis was induced by given retinoic acid intragastrically.After oral administration for14days,the features of bones and biochemicals in serum were determined to evaluate the anti-osteoporosis effects of PM-HE.The results showed:PM-HE can induce high conversion of osteoporosis.The levels of ALP,TRAP and BGP in the PM-HE groups were lower than those in the model group(P<0.05,P<0.01),whereas the E2level was higher than that of the model group(P<0.05).PM-HE can prevent the loss bone in the experimental mice.The mechanism may be the inhibition of estrogen decrease and the high bone turnover.
作者
吴晓青
陈晓珍
刘睿颖
任瑶瑶
蒋合众
谭睿
WU Xiao-qing;CHEN Xiao-zhen;LIU Rui-ying;REN Yao-yao;JIANG He-zhong;TAN Rui(College of life science and Engineering,Southwest Jiao Tong University ,Chengdu 610031 ,China;Institute of Biology,Chinese Academy of Sciences,Chengdu, 610041 ,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2018年第12期2175-2179,共5页
Natural Product Research and Development
基金
四川省中医药管理局科研项目(2016C050)
四川省重点研发项目(2017SZ0075)
国家自然科学基金(21402187)
关键词
生首乌
制首乌
维甲酸
抗骨质疏松
raw Polygonum multiflorum Thunb
processed Polygonum multiflorum
retinoic acid
anti-osteoporosis
