摘要
为从分子水平研究双孢蘑菇多酚氧化酶调控褐变的机理,以采后贮藏第1、7、14天的双孢蘑菇菌盖为材料,应用转录组测序技术对双孢蘑菇菌盖RNA进行测序分析。共获得168 607 212个高质量的clean reads;贮藏第7天和第1天相比筛选出727个差异表达基因(differentially expressed genes,DEGs),贮藏第14天和第1天相比筛选出1 524个DEGs;Gene Ontology(GO)功能富集和Pathway富集分析将这些差异表达基因归类于180个代谢途径;从已获得的差异表达基因中筛选得到PPO1、PPO3、PPO5、LAC1和LAC4五个多酚氧化酶相关基因。研究结果为双孢蘑菇功能相关基因挖掘和品质改良提供理论数据支撑。
In order to study how polyphenol oxidase regulates the progression of Agaricus bisporus browning, we performed mRNA sequencing using the RNA-seq technique on the pilei of A. bisporus collected on day 1, 7 and 14 of postharvest storage. A total of 168 607 212 high-quality reads were obtained; 727 and 1 524 differentially expressed genes were identified on day 7 and 14 compared with day 1 of storage, respectively. These differentially expressed genes were classified into 180 metabolic pathways after gene ontology and pathway enrichment analysis; of them, five polyphenol oxidase genes (PPO1, PPO3, PPO5, LAC1 and LAC4) were screened out and their relationships with browning were investigated. These results provide a theoretical basis for mining of functional genes and quality improvement of A. bisporus.
作者
彭博
李炳娟
关文强
林琼
PENG Bo;LI Bingjuan;GUAN Wenqiang;LIN Qiong(Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China;Institute of Agro-Products Processing Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第2期126-132,共7页
Food Science
基金
天津市自然科学基金重点项目(16JCZDJC3400)
国家自然科学基金面上项目(31271949)
天津市高等学校创新团队建设项目(TD13-5087)
关键词
双孢蘑菇
褐变
转录组测序技术
差异表达基因
多酚氧化酶
Agaricus bisporus
browning
RNA-seq
differentially expressed genes(DEGs)
polyphenol oxidase(PPO)
