摘要
目的探讨长链非编码RNA TUSC8调控miR-137对宫颈癌迁移和侵袭的影响及其机制。方法 qPCR检测TUSC8和miR-137在宫颈癌组织和正常宫颈组织中的表达情况及差异,分析TUSC8与宫颈癌患者临床病理学参数之间的相关关系,双荧光素酶报告基因检测TUSC8与miR-137之间的相互作用;Transwell侵袭实验检测抑制TUSC8后宫颈癌细胞侵袭行为的变化情况;划痕愈合实验检测抑制TUSC8后宫颈癌细胞迁移行为的变化情况;裸鼠体内成瘤实验抑制TUSC8后宫颈癌细胞的成瘤能力的变化。结果与正常宫颈组织相比,宫颈癌组织中TUSC8和miR-137的表达水平均相对上调,差异有统计学意义(P<0.05);TUSC8的表达与宫颈癌的病理分期相关以及淋巴结转移情况有关,分期越高,TUSC8在宫颈癌组织中表达越高,淋巴结转移的患者中TUSC8的表达也相对较高;双荧光素酶实验证实TUSC8能与miR-137的3’UTR特异性结合,可以调控miR-137的表达与活性;抑制TUSC8的表达后可以抑制宫颈癌细胞的迁移和侵袭能力;抑制TUSC8后宫颈癌细胞的成瘤能力受到相应的抑制。结论 TUSC8可以调控miR-137的表达影响宫颈癌细胞的迁移和侵袭行为。
Objective To investigate the effect of long-chain non-coding RNA TUSC8 on the migration and invasion of cervical cancer and its mechanism. Methods qPCR was used to detect the expression and differences of TUSC8 and miR-137 in cervical cancer tissues and normal cervical tissues. The correlation between TUSC8 and clinicopathological parameters in patients with cervical cancer was analyzed. Double luciferase reporter gene was used to detect the interaction between TUSC8 and miR-137. The invasion of TUSC8 cells was detected by Transwell invasion assay. Scaffold healing test was used to detect the changes of migration behavior of TUSC8 after cervical cancer cells. Inhibitory effect of TUSC8 on the tumorigenesis of cervical cancer cells in nude mice by tumorigenesis. Results Compared with normal cervical tissues, the expression levels of TUSC8 and miR-137 in cervical cancer tissues were up-regulated. The difference was statistically significant. The expression of TUSC8 was related to the pathological stage of cervical cancer and lymph node metastasis. The higher the expression of TUSC8 in cervical cancer, the higher the expression of TUSC8 in patients with lymph node metastasis. Double luciferase assay confirmed that TUSC8 could bind to 3 'UTR of miR-137 and could regulate the expression and activity of miR-137. Inhibition of TUSC8 expression could inhibit the migration and invasion of cervical cancer cells. The posterior cervical cancer cell tumorigenic ability decreases after inhibition of TUSC8. Conclusion TUSC8 can regulate the expression of miR-137 and affect the migration and invasion of cervical cancer cells.
作者
田延龙
何小岗
高晓
田伟
杨学超
TIAN Yanlong;HE Xiaogang;GAO Xiao;TIAN Wei;YANG Xuechao(Department of Pathology, No.215 Hospital of Shanxi Nuclear Industry, Xianyang 712000, Shanxi, China)
出处
《西部医学》
2019年第1期24-29,共6页
Medical Journal of West China
基金
陕西省科学计划项目(2012K15-02)
