摘要
目的:建立雄性昆明小鼠H22肝癌移植瘤动物模型,观察山仙颗粒对H22肝癌移植瘤的抑制作用及对Caspase-3、MST1表达的影响,以探讨山仙颗粒对H22移植瘤细胞凋亡的调控作用。方法:建立雄性昆明小鼠H22肝癌移植瘤动物模型,随机分为5组,即空白组、模型组、山仙颗粒大、中、小剂量组。早晚灌胃给药各一次,连续30天。颈椎脱臼法处死小鼠,剥离瘤组织、称重,计算抑瘤率。SABC免疫组织化学法检测移植瘤组织中Caspase-3及MST1的表达。结果:山仙颗粒大、中、小剂量组对H22肝癌的抑瘤率分别为41. 246%、26. 801%、19. 835%,抑瘤率与用药剂量呈正相关(P <0. 01);山仙颗粒大、中、小剂量组分别与模型组比较Caspase-3、MST1的表达均增强,其表达强度与药物剂量呈正相关(P <0. 01)。结论:山仙颗粒具有抑制小鼠H22肝癌移植瘤生长的作用,其机制可能与其通过调控Caspase-3/MST1通路而诱导肿瘤细胞凋亡相关。
Objective:To establish an animal model of H22 hepatocarcinoma xenografts in male Kunming mice and observe the inhibitory effect of Shan Xian granule on H22 liver cancer and its effect on the expression of Caspase-3 and MST1,to explore the effect of Shan Xian granule on the apoptosis of H22 hepatocarcinoma xenografts.Methods:Male Kunming mice were established as H22 hepatoma xenograft tumor models and randomly divided into 5 groups,including blank group,model group,large,medium and small doses of Shan Xian granules groups,administered intragastrically each morning and evening for 30 days.The mice were sacrificed by cervical dislocation.Their tumor was removed and weighed.The tumor suppressor rate was calculated.SABC immunohistochemistry was applied to detect the expression of Caspase-3 and MST1 in transplanted tumor tissues.Results:The tumor inhibition rates of H22 hepatocellular carcinoma in large,medium and small doses of Shan Xian granules were 41.246%,26.801%and 19.835%respectively.The tumor inhibition rate was positively correlated with the dose(P<0.01).Compared with the model group,the expressions of Caspase-3 and MST1 in the large,medium and small doses of Shan Xian granules were significantly enhanced.The expression level was positively correlated with the dose(P<0.01).Conclusion:Shan Xian granules can inhibit the proliferation of H22 hepatocarcinoma xenografts,and its mechanism may be related to the apoptosis of tumor cells induced by the regulation of Caspase-3/MST1 pathway.
作者
应小平
胥冰
方艳
潘艳芳
李宏
焦佩娟
芮冉
董夏
孟博博
Ying Xiaoping;Xu Bing;Fang Yan;Pan Yanfang;Li Hong;Jiao Peijuan;Rui Ran;Dong Xia;Meng Bobo(Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712046,China.)
出处
《现代肿瘤医学》
CAS
2019年第5期715-719,共5页
Journal of Modern Oncology
基金
国家自然科学基金项目(编号:81703842)
陕西省中管局科研计划项目(编号:JCMS032)
