摘要
本研究以全反式、9-顺式和13-顺式虾青素为研究对象,采用体内外相结合的方法,揭示不同几何构型虾青素的抗氧化作用和对氧化应激损伤的影响。结果表明:在清除1,1-二苯基-2-三硝基苯肼自由基方面,3种不同几何构型虾青素之间均没有显著差异(P>0.05);在清除2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸自由基方面,9-顺式虾青素显著强于13-顺式和全反式虾青素(P<0.05);在氧化自由基吸收能力方面,9-顺式虾青素显著高于13-顺式虾青素(P<0.05),13-顺式虾青素和全反式虾青素之间没有显著差异(P>0.05);在二氯百草枯诱导的氧化应激条件下,3种几何构型虾青素均能显著提高秀丽隐杆线虫的存活率(P<0.05),但它们之间无显著差异(P>0.05);与对照组相比,9-顺式和全反式虾青素显著降低线虫体内活性氧的积累量(P<0.05),分别下降56.05%和15.07%,9-顺式虾青素处理组与13-顺式虾青素处理组、全反式虾青素处理组均有显著性差异(P<0.05)。因此,3种几何构型虾青素均具有体外抗氧化作用和对氧化应激损伤的保护作用,9-顺式虾青素的活性优于全反式和13-顺式虾青素。
In this study,the in vitro antioxidant activity of different geometric isomers of astaxanthin(all-trans,9-cis,and 13-cis)was studied as well as the protective effect against oxidative stress in vivo.The results showed no significant difference among these geometric isomers of astaxathin(P﹥0.05)in 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging capacity,but in terms of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)free radical scavenging capacity,9-cis astaxanthin was significantly better than 13-cis and all-trans astaxanthin(P﹤0.05);in terms of total antioxidant capacity,9-cis astaxanthin was significantly better than 13-cis astaxanthin(P﹤0.05)although there was no significant difference between 13-cis and all-trans astaxanthin(P﹥0.05).Under the condition of oxidative stress induced by paraquat(PQ),all geometric isomers of astaxathin could significantly increase the survival rate of Caenorhabditis elegans(P﹤0.05)and their effects were not significantly different(P﹥0.05).Compared with the control group,9-cis and all-trans astaxanthin could significantly reduce the accumulation of reactive oxygen species(ROS)in nematodes by 56.05%and 15.07%,respectively(P﹤0.05),and there was a significant difference between the 9-cis group and other treatment groups(P﹤0.05).Therefore,all three geometric isomers of astaxathin exhibit antioxidant activity and protection against oxidative stress,and 9-cis astaxathin has better antioxidant activity than all-trans and 13-cis astaxanthin.
作者
刘涵
陈晓枫
刘晓娟
贺丽苹
曹庸
LIU Han;CHEN Xiaofeng;LIU Xiaojuan;HE Liping;CAO Yong(College of Food Science,South China Agricultural University,Guangzhou 510642,China;Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods,Guangzhou 510642,China;Test Center,South China Agricultural University,Guangzhou 510642,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第3期178-185,共8页
Food Science
基金
广州市科技计划项目(201707010035)
广东省自然科学基金项目(2017A030313150)
国家自然科学基金青年科学基金项目(31401481)
关键词
虾青素
几何构型
抗氧化
氧化应激
秀丽隐杆线虫
astaxanthin
geometric isomer
antioxidant
oxidative stress
Caenorhabditis elegans
