摘要
目的观察温阳补肾方对兔激素性股骨头坏死(SANFH)组织中破骨细胞抑制因子(OPG)、核因子-κB受体活化因子(RANK)和核因子-κB受体活化因子配体(RANKL)的mRNA表达的影响。方法普通级健康新西兰大白兔46只,分为正常组(n=10)和造模组(n=36)。应用改进马血清联合甲基强的松龙法制作SANFH模型。造模后随机选取正常组2只、造模组4只处死,用于模型鉴定。再将造模组剩余动物随机分为模型组(n=8),中药低(n=8)、中(n=8)和高(n=8)剂量组。正常组和模型组生理盐水10 ml/d灌胃。中药低、中、高剂量组分别为以6.44 g/(kg·d)、9.66 g/(kg·d)、12.88 g/(kg·d)灌胃温阳补肾方汤剂,连续8周。采用逆转录聚合酶链反应检测OPG、RANK和RANKL的mRNA表达。结果模型组空骨陷窝率显著高于正常组(t=17.085, P <0.001)。与模型组比较,中药各剂量组OPG mRNA表达均明显升高(P <0.01),RANK和RANKL的mRNA表达明显降低(P <0.01);与中药低剂量组比较,中药中、高剂量组OPG mRNA表达明显升高(P <0.01),RANK和RANKL的mRNA表达明显降低(P <0.01);中药中、高剂量组比较均无显著性差异(P> 0.05)。结论温阳补肾方能提高兔SANFH股骨头组织中OPG的mRNA表达,抑制RANK和RANKL的mRNA表达。
Objective To observe the effect of Wenyangbushen formula on mRNA expression of osteoprotegerin (OPG), receptor activator of nuclear factor-kappa B (RANK) and receptor activator of nuclear factor-kappa B ligand (RANKL) in rabbits with steroid-induced avascular necrosis of femoral head (SANFH). Methods A total of 46 healthy conventional New Zealand white rabbits were randomly divided into normal group ( n = 10) and model building group ( n = 36). The modified method of horse serum plus methylprednisolone was used to establish the SANFH model. Two rabbits from the normal group and four from the model building group were used for HE staining. Then the other models were randomly divided into model group, and low-dose, medium-dose and high-dose treatment groups, with eight rabbits in each group. The normal group was given normal saline 10 ml/d, and the treatment groups were given Wenyangbushen formula 6.44 g/(kg · d), 9.66 g/(kg · d) and 12.88 g/(kg · d), respectively, for eight weeks. The mRNA expression of OPG, RANK and RANKL was detected by reverse transcription-polymerase chain reaction. Results The empty lacuna rate was significantly higher in the model group than in the normal group ( t = 17.085, P < 0.001). Compared with the model group, the mRNA expression of OPG increased ( P < 0.01), and the mRNA expression of RANK and RANKL decreased ( P < 0.01), in the treatment groups. Compared with the low-dose treatment group, the mRNA expression of OPG increased ( P < 0.01), and the mRNA expression of RANK and RANKL decreased ( P < 0.01), in the medium-dose and high-dose treatment groups. There was no significant difference in the mRNA expression of OPG, RANK and RANKL between the low-dose treatment group and the high-dose treatment group ( P > 0.05). Conclusion Wenyangbushen formula could increase the mRNA expression of OPG and inhibit the mRNA expression of RANK and RANKL in the femoral head tissue of the rabbits with SANFH.
作者
宋红梅
魏迎辰
李楠
王和鸣
SONG Hong-mei;WEI Ying-chen;LI Nan;WANG He-ming(The Second People's Hospital of Fujian Province,Fuzhou,Fujian 350003,China;The Second Affiliated Hospital of Anhui University of Chinese Medicine,Hefei,Anhui 230061,China;Fujian University of Traditional Chinese Medicine, Fuzhou,Fujian 350122,China)
出处
《中国康复理论与实践》
CSCD
北大核心
2019年第2期178-183,共6页
Chinese Journal of Rehabilitation Theory and Practice
基金
国家自然科学基金面上项目(No.81173283)
福建省自然科学基金卫生联合项目(No.2016J01560)
福建省卫生计生中青年骨干人才培养项目(No.2016-ZQN-73)~~
关键词
激素性股骨头坏死
温阳补肾方
骨保护素
核因子-κ
B受体活化因子
核因子-κ
B受体活化因子配体
兔
steroid-induced avascular necrosis of femoral head
Wenyangbushen formula
osteoprotegerin
receptor activator of nuclear factor-kappa B
receptor activator of nuclear factor-kappa B ligand
rabbits
