摘要
目的·研究枸杞多糖(Lycium barbarum polysaccharide,LBP)对髓样分化因子88(myeloid differentiation factor 88,MyD88基因敲除(MyD88-/-)小鼠2型糖尿病(type 2 diabetes mellitus,T2DM)模型炎症因子的影响。方法·采用不同浓度LBP(20、40、80 mg/kg)干预MyD88-/-T2DM小鼠,ELISA法检测小鼠血清中白细胞介素1β(IL-1β)、IL-6、IL-8、转化生长因子β1(TGF-β1)及IL-10的水平。采用不同浓度(25、50、100μg/mL)LBP预处理小鼠巨噬细胞系Raw264.7细胞,然后用脂多糖诱导炎症状态,Western blotting检测各组细胞核因子κB(NF-κB)的核转位变化,以及NF-κB抑制蛋白(IκB)和磷酸化IκB的蛋白水平。结果·LBP能够降低MyD88-/-T2DM小鼠血清IL-1β和TGF-β1的水平(均P<0.05)。体外实验表明,LBP可以剂量依赖性地抑制巨噬细胞中由脂多糖诱发的NF-κB核转位,同时高剂量的LBP可以抑制IκB磷酸化。结论·LBP可以抑制MyD88-/-T2DM小鼠部分促炎症因子,这种调节作用可能与其弱化巨噬细胞IκB磷酸化,抑制NF-κB核转位有关。
Objective · To investigate the effect of Lycium barbarum polysaccharides on inflammatory cytokines in type 2 diabetes mellitus (T2DM) mice without myeloid differentiation factor 88 gene (MyD88-/-). Methods · Levels of interleukin 1β(IL-1β), IL-6, IL-8, transforming growth factor β1 (TGF-β1), and IL-10 in serum were assessed by ELISA in the MyD88-/- T2DM mice which had been administered with different doses of LBP (20, 40, and 80 mg/kg). Mouse macrophages Raw264.7 were stimulated by lipopolysaccharide (LPS) after treatment with different concentrations of LBP (25, 50, and 100 μg/mL). Then Western blotting was used to detect nuclear translocation level of nuclear factor κB (NF-κB) and protein expressions of inhibitor of NF-κB (IκB) and p-IκB. Results · Serum levels of IL-1β and TGF-β1 in MyD88-/- T2DM mice were down-regulated by LBP (P<0.05). Cell experiment proved that nuclear migration of NF-κB was dose-dependently inhibited by LBP, and the level of p-IκB was reduced by high dose of LBP. Conclusion · LBP can reduce some proinflammatory cytokines in the MyD88-/- T2DM mice, which may be related with its inhibitive effect on the phosphorylation of IκB and nuclear migration of NF-κB in the macrophages.
作者
王凌霄
刘婷婷
杨晓辉
姚智卿
蔡慧珍
WANG Ling-xiao;LIU Ting-ting;YANG Xiao-hui;YAO Zhi-qing;CAI Hui-zhen(School of Public Health & Management,NingXia Medical University,Yinchuan 750004,China)
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2019年第2期136-141,共6页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(81460494)
宁夏高等学校科学研究项目(NGY2013072)
2017年宁夏医科大学青年学术骨干人才后备培养项目~~
关键词
枸杞多糖
髓样分化因子88
基因敲除
2
型糖尿病
巨噬细胞
炎症因子
核因子κ
B
Lycium barbarum polysaccharide (LBP)
myeloid differentiation factor 88 (MyD88)
gene knockout
type 2 diabetes mellitus (T2DM)
macrophage
inflammatory cytokine
nuclear factor κB (NF-κB)
