摘要
目的研究+5 kV驻极体、5-氟尿嘧啶(5-FU)贴剂和+5 kV驻极体5-FU贴剂对兔耳瘢痕组织中Ⅰ、Ⅲ型胶原和转化生长因子-β(TGF-β)表达的影响。方法将通过电晕充电系统制备的正极性聚丙烯驻极体充电面与制备的5-FU贴剂背衬层结合制成表面电位为+5 kV的驻极体5-FU贴剂,再将+5 kV驻极体、5-FU贴剂和+5 kV驻极体5-FU贴剂分别作用于兔耳创面,通过测算瘢痕增生指数,观察研究瘢痕增生状况,通过HE染色、Masson染色和免疫组织化学的方法,研究上述驻极体、5-FU贴剂和驻极体5-FU贴剂对兔耳瘢痕组织中Ⅰ、Ⅲ型胶原和TGF-β表达的影响。结果①兔耳创面自然愈合4周后形成增生性瘢痕组织。②正极性驻极体不会导致创面过度修复。③5-FU贴剂和正极性驻极体5-FU贴剂通过减少瘢痕组织内Ⅰ型胶原、Ⅲ型胶原和TGF-β的表达量,抑制增生性瘢痕的生长。④正极性驻极体5-FU贴剂抑制增生性瘢痕生长的效果优于5-FU贴剂。结论正极性驻极体与5-FU联用抑制增生性瘢痕生长的效果更好。
Objective To study the effects of positive electret 5-FU patch on the expression of typeⅠ,Ⅲcollagens and TGF-βin rabbit ear scar tissue.Methods The positive electret 5-fluorouracil(5-FU)patches with surface potential of+5 kV were made by attaching the positive charged polypropylene(PP)electret on the back layer of the 5-FU patch.The positive electrets,5-FU patch and positive electret 5-FU patches were applied to the wound surface on rabbit ear.The scar proliferation index was calculated to study the status of the scar.the HE staining,Masson staining and immunohistochemical methods were used to study the effects of the positive electrets,5-FU patch and the positive electret 5-FU patch on the expression of typeⅠ,Ⅲcollagen and TGF-βin the rabbit ear scar tissue.Results①The hypertrophic scar(HS)was formed after 4 weeks natural healing.②The positive electret will not lead to excessive wound repair.③Both the 5-FU patch and the positive electret 5-FU patch reduced the expression of typeⅠcollagen,typeⅢcollagen and TGF-βin scar tissue and inhibited the growth of HS.④The positive electret 5-FU patch had better inhibiting effect in HS growth than 5-FU patch.Conclusion The combination of positive electret and 5-FU can achieve better results in inhibiting the growth of hypertrophic scar.
作者
安晓强
苑旺
梁媛媛
郭鑫
梁合鹃
江键
崔黎丽
AN Xiaoqiang;YUAN Wang;LIANG Yuanyuan;GUO Xin;LIANG Hejuan;JIANG Jian;CUI Lili(Department of Inorganic Chemistry,School of Pharmacy,Naval Medical University,Shanghai 200433,China;Department of Physics and Mathematics,Faculty of Health Service,Naval Medical University,Shanghai 200433,China)
出处
《药学实践杂志》
CAS
2019年第2期115-120,145,共7页
Journal of Pharmaceutical Practice
基金
国家自然科学基金面上项目(51477175)
