摘要
目的比较高氯酸滴定法与高效液相色谱(HPLC)法测定地巴唑片中地巴唑的含量。方法高氯酸滴定法中以甲基橙为指示液;HPLC法,色谱柱为Agilent Eclipse XDB-C_(18)柱(250 mm×4. 6 mm,5μm),流动相为甲醇-水-三乙胺(80∶19. 5∶0. 5,V/V/V),流速为1. 0 mL/min,检测波长为275 nm,柱温为26℃,进样量为10μL。结果高氯酸滴定法测定的平均含量为94. 85%,RSD为3. 50%(n=5); HPLC法测定的平均含量为97. 80%,RSD为1. 48%(n=5)。结论 HPLC法能更好地减少杂质干扰,可更准确地测定地巴唑片中地巴唑的含量。
Objective To compare the content determination of bendazol in Tabellae Bendazoli by perchloric acid titration or HPLC. Methods Helianthin B was taken as the indicator solution in perchloric acid titration. Agilent Eclipse XDB-C18 column( 250 mm × 4. 6 mm,5 μm) was adopted by HPLC,the mobile phase was methanol-water-triethylamine( 80 ∶ 19. 5 ∶ 0. 5, V/V/V),the flow rate was 1. 0 mL/min,the detection wavelength was 275 nm,the column temperature was 26 ℃,and the sample size was 10 μL.Results The average content of bendazol in Tabellae Bendazoli by perchloric acid titration was 94. 85%, RSD was 3. 50%( n = 5),while the average content of bendazol in Tabellae Bendazoli by HPLC was 97. 80%, RSD was 1. 48%( n = 5). Conclusion HPLC can reduce the interference of impurities better,and determine the content of bendazol in Tabellae Bendazoli more accurately.
作者
陆广马
LU Guangma(Baise Institute for Food and Drug Control,Boise,Guangxi,China 533000)
出处
《中国药业》
CAS
2019年第8期19-21,共3页
China Pharmaceuticals
基金
广西壮族自治区百色市科学研究与技术开发计划课题[百科计20150316]
