摘要
【目的】α-溶血素在金黄色葡萄球菌(金葡菌)肺炎的感染过程中起着非常重要的作用,可作为治疗金葡菌肺炎的新靶标。因此,研究百里香酚对金葡菌α-溶血素表达的影响具有重要意义。【方法】测定最小抑菌质量浓度(MIC)和生长曲线并进行了溶血试验、蛋白免疫印迹分析、荧光定量PCR以及细胞毒性试验。【结果】百里香酚对金葡菌USA300的MIC为256μg/mL,亚抑菌质量浓度的百里香酚(32μg/mL)可下调金葡菌hla和RNAIII的转录水平,降低α-溶血素的表达,对溶血素介导的肺癌上皮细胞A549损伤具有保护作用。【结论】百里香酚具有成为新型抗金葡菌感染药物的潜力。
[Purpose]Alpha-hemolysin (Hla) plays a significant role in the process of Staphylococcus aureus pneumonia. Hla is regarded as a novel target for the treatment of S. aureus infection.[Method]The inhibitory effect of thymol against S. aureus Hla was studied via determining the ef- fects of thymol on the growth of S. aureus and hemolysis assay, Western-blot, Real-time PCR and cytotoxicity assay.[Result] The MIC of thymol against S. aureus was 256 μg/mL. Thymol can down-regulate the transcriptional levels of hla and RNAIII, inhibited the expression of Hla. Thymol protected A549 cells injure induced by S. aureus Hla.[Conclusion]Our results showed that thymol has the potential to be developed a novel drug for S. aureus pneumonia targeting Hla.
作者
孙茂
何学文
邓嘉强
徐悦
王君锐
尹立子
SUN Mao;HE Xuewen;DENG Jiaqiang;XU Yue;WANG Junrui;YIN Lizi(College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2019年第3期414-419,共6页
Journal of Yunnan Agricultural University:Natural Science
基金
四川农业大学双支计划(03571444)
四川省教育厅青年基金(16ZB0036)
