摘要
目的观察Wnt信号通路在Aβ25-35诱导PC12细胞凋亡中的作用。方法Aβ25-35模拟PC12细胞氧化应激损伤,WST-1法检测PC12细胞的增殖活力,倒置显微镜观察细胞的形态,流式细胞仪检测细胞的凋亡率,Western Blot检测Bax/Bcl-2表达量的变化。结果在一定浓度范围内,Wnt3a呈浓度依赖的增加PC12细胞存活率,改善细胞形态,减少早期凋亡细胞数目,升高细胞Bcl-2/Bax比值,且浓度100 ng/ml时达到最佳效应。结论Wnt3a可能通过Wnt信号通路抑制过氧化氢(H2O2),诱导线粒体凋亡通路,进而发挥保护由Aβ25-35诱导的PC12细胞损伤。
【Objective】 To observe the effects of Wnt3a on Aβ25-35 inducing PC12 cells apoptosis.【Methods】 PC12 oxidative-stress damage was induced by 30μM Aβ25-35, cell survival rate was determined by WST-1, cell morphology was observed by the inverted microscope, cell apoptosis was detected by flow cytometry, and the change of Bax/Bcl-2 was examined by westem-blot.【Results】 The cell survival rate was determined by the WST-1 method during the process of PC 12 cell cultivation. The survival rate of PC12 cells became higher with the concentration of Wnt3a increasing, the apoptosis rate of PC12 cells became lower with the concentration of Wnt3a increasing, especially at the concentration of 100 ng/ml. Wnt3a in 100 ng/ml reaches its protective effect peak and can improve cell morphology obviously, reduce the quantity of early apoptotic cells and rise the ratio of Bcl-2/ Bax.【Conclusion】 Wnt3a has the protective effect on Aβ25-35 induced PC12 dells damage,the mechanism may be related to the inhibition on H2O2-induced mitochondrial apoptotic pathways by Wnt3a via Wnt signal pathway.
作者
吴国访
张淑沛
WU Guofang;ZHANG Shupei(Department of Neurology,the Sixth People's Hospital ofLuohe City,Luohe,Henan 462002,China)
出处
《中国医学工程》
2019年第4期8-11,共4页
China Medical Engineering
