摘要
为明确甜瓜种子携带黄瓜花叶病毒(Cucumher mosaic virus,CMV)状况,以5个品种甜瓜种子为材料,采用实时荧光定量PCR技术检测甜瓜种子、胚芽中CMV带毒率和带毒量。结果表明,供试种子、种胚带毒率分别为80%~96.6%和5.0%~25.0%,带毒量分别为6.62×10^4~5.47×10^7拷贝/粒和3.78×10^4~1.00×10^6拷贝/粒;种苗传毒率为0~6.6%,不同品种之间存在差异。种子的带毒率高而传毒率低,表明CMV主要存在于甜瓜种子的种皮上。试验比较了5种处理方法对带CMV甜瓜种子的脱毒效果。干热(65℃)、湿热(100℃沸水)、温汤(55℃)、干热+湿热(65℃干热+100℃沸水)、干热+10%Na 3PO4(65℃干热+10%Na3PO4)处理甜瓜种子,均能降低种子带毒量。干热、湿热、干热+湿热和干热+10%Na3PO4处理后种子脱毒率可达96%以上,显著优于温汤处理(P<0.05),并能促进种子发芽。干热+10%Na 3PO4处理甜瓜种子后的种苗中未检出病毒,对CMV的脱除效果最佳。
The study aims to determine melonseeds infected by Cucumber mosaic virus(CMV),the infection rate of seed,plumule,and seed-to-seedling transmission of CMV in five melon varieties were detected,and the effect of five treatments on the virus elimination were evaluated by using fluorescence real-time RT-PCR method.The results showed that the infection ratesof seeds and plumule infected by CMVwere 80%-96.6%and 5.0%-25.0%,the amounts of the virus were 6.62×10^4-5.47×10^7 and 3.78×10 4-1.00×10^6 copies/one seed,respectively.The seed-to-seedling transmission ratesbetween 0-6.6%were different depended on the varieties of melon seed.The transmission rate of seed-to-seedling was low while the infection rate was high,this indicated that the virus mainly distributed on the surface of the seeds.The effects of five treatments(65℃dry heat,100℃wet heat,55℃wet heat,65℃dry heat+100℃wet heat,65℃dry heat+10%Na 3PO 4)on virus elimination varied.The elimination efficiency of CMV was more than 96%when the seeds were treated by 65℃dry heat,100℃wet heat,65℃dry heat+100℃wet heat and 65℃dry heat+10%Na 3PO 4,also these treatments could promote the seed germination.The treatment of dry heat and 10%Na 3PO 4 showed that the virus detection rate of the seedling was zero,which was the most effective method for removing CMV from the melon seeds.
作者
韩剑
肖力
罗明
潘亚南
宁轩
HAN Jian;XIAO Li;LUO Ming;PAN Yanan;NING Xuan(College of Agronomy,Xinjiang Agricultural University,Urumqi830052,China;Key Laboratory of Prevention and Controlfor Pest Monitoring and Security of Autonomous Universities in Xinjiang,Urumqi830052,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2019年第5期774-781,共8页
Acta Agriculturae Boreali-occidentalis Sinica
基金
农业部西北荒漠绿洲作物有害生物综合治理重点实验室开放基金(KFJJ20150103)
国家级大学生创新创业训练计划(201610758002)~~
关键词
甜瓜
黄瓜花叶病毒
REAL-TIMEPCR
传毒率
脱毒
Melon
Cucumber mosaic virus
Real-time PCR
Seed-to-seedling transmission rate
Virus elimination
