摘要
目的探讨黑骨藤追风活络胶囊对胶原诱导型关节炎(CIA)大鼠核因子κB(NF-κB)相关蛋白的影响。方法雄性SD大鼠随机分为对照组、模型组、黑骨藤追风活络胶囊0.315 g/kg组、 0.315 g/kg黑骨藤追风活络胶囊联合0.01 g/kg NF-κB抑制剂吡咯烷二硫代氨基甲酸铵(PDTC)处理组。除对照组外,其余各组建立CIA模型。给药5周后,厚度测量仪测量大鼠足跖厚度,计算脾脏指数(脾脏质量/体质量),免疫组织化学染色法检测脾脏转化生长因子活化激酶1(TAK1)、转化生长因子β活化激酶结合蛋白1(TAB1)的表达, Western blot法检测关节滑膜组织NF-κB抑制蛋白α(IκBα)、核因子κBp65亚基(NF-κBp65)、磷酸化核因子κBp65亚基(p-NF-κBp65)、 NF-κB受体激活蛋白配体(RANKL)蛋白水平, ELISA检测血浆单核细胞趋化蛋白1(MCP-1)、 CXC趋化因子配体1(CXCL1)、巨噬细胞炎性蛋白1(MIP-1)水平。结果与对照组相比,模型组大鼠足跖肿胀度、脾脏指数明显增加;与模型组相比,黑骨藤追风活络胶囊组和联合给药组大鼠足跖肿胀度和脾脏指数明显降低; TAK1和TAB1蛋白表达下调,滑膜组织IκBα蛋白水平增加, NF-κBp65、 p-NF-κBp65、 RANKL蛋白水平降低;血浆MCP-1、 CXCL1、 MIP-1水平下降。结论黑骨藤追风活络胶囊抑制NF-κB通路相关蛋白活性减轻CIA大鼠炎症反应。
Objective To investigate the effect of Heiguteng Zhuifenghuoluo Capsule(HZC) on nuclear factor kappa B(NF-κB)-related protein in rheumatoid arthritis rats, and to analyze the expression of IκBα, NF-κBp65, phospho-nuclear factor kappa B p65(p-NF-κBp65), receptor activator of nuclear factor κB ligand(RANKL), transforming growth factor activated kinase 1(TAK1), transforming growth factor β activated kinase binding protein 1(TAB1), monocyte chemoattractant protein 1(MCP-1), C-X-C motif chemokine ligand 1(CXCL1), macrophage inflammatory protein-1(MIP-1) of plasma in rheumatoid arthritis rats. Methods Male SD rats were randomly divided into control group, model group, HZC(0.315 g/kg) group, and HZC(0.315 g/kg) plus ammonium pyrrolidinedithiocarbamate(PDTC, 0.01 g/kg) group. The model was constructed in all groups except the control group. Five weeks after modeling, the plantar thickness and spleen organ coefficient(spleen/body weight ratio) were measured in all rats. The TAK1 and TAB1 proteins were detected by immunohistochemical method in the spleen. Western blot analysis was used to detect the expression of IκBα, NF-κBp65, p-NF-κBp65 and RANKL proteins in the synovium of joint and plantar tissue. MCP-1, CXCL1, MIP-1 expression in the plasma was detected by ELISA. ResultsCompared with the model group, the swelling degree of plantar and the spleen organ coefficient in the control group, HZC group and combined administration group decreased significantly. The immunohistochemistry showed that the expression of TAK1 and TAB1 proteins in the spleen were down-regulated in the control group, HZC group and combined administration group. Western blotting showed that the expression of IκBα protein was up-regulated in the synovium of joint tissue of the control group, HZC group and combined administration group, but the expression of NF-κBp65, p-NF-κBp65, RANKL proteins were down-regulated in the control group, HZC group and combined administration group. ELISA showed that the expression of plasma MCP-1, CXCL1, MIP-1 were down-regulated in the control group, HZC group and combined administration group. Conclusion HZC inhibits the inflammatory response of rheumatoid arthritis rats by regulating NF-κB-related proteins.
作者
梁江
王乾宇
黄维琛
杨长福
潘春
陈向云
刘杨
LIANG Jiang;WANG Qianyu;HUANG Weichen;YANG Changfu;PAN Chun;CHEN Xiangyun;UU *Yang(Guizhou University of Chinese Medicine, Guiyang 550025 , China)
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2019年第3期193-198,共6页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(81560817)
贵州省一流学科建设项目(QYNYL[2017]005)
贵州省科技厅项目(黔科合平台人才[2017]5735号-08
黔科合J字[2013]2074号
黔教合KY字[2015]438号)
贵州省重大基础专项项目(苗药协同创新中心)([2015]05号)
