摘要
目的:客观评价已建立的滴鼻途径感染致肺炎大鼠模型,为后续肺炎防治机制研究提供实验依据。方法:将24只SD雄性大鼠按随机数表法分为对照组和实验组,每组12只,两组均按干预后0d、5d、10d和15d分为4个亚组,每组3只。以滴鼻途径干预,采集干预0d、5d、10d和15d的各亚组肺泡灌洗液(BALF)及肺组织样本,检测其肺指数、BALF中白细胞数、免疫炎性因子IL-4、TNF-α和IFN-γ的表达及铜绿假单胞菌数量,并分别与对照组的相应亚组比较分析。结果:实验组大鼠肺指数在造模干预后,随时间延长5d、10d和15d逐渐增大,与对照组相比差异有统计学意义(t=-2.46,t=-3.26,t=-3.73;P<0.05);BALF中白细胞数均明显高于相应对照亚组,BALF中IL-4和TNF-α在造模干预后均呈增长趋势,两组比较差异有统计学意义(t=4.24,t=4.88;P<0.05),IFN-γ则呈下降趋势;BALF中铜绿假单胞菌数在造模干预后,随时间延长呈增多趋势。结论:成功构建的滴鼻感染耐药铜绿假单胞菌致大鼠肺炎模型,可用于中医药防治肺炎的机制研究。
Objective: To evaluate objectively the established rat pneumonia model caused by intranasal inoculation so as to provide experimental basis for the followed mechanism study of prevention and treatment of pneumonia. Methods: 24 SD male rats were divided into control group (n=12) and experimental group randomly (n=12), and each group was further divided into 4 subgroups as 0, 5, 10 and 15 days post intervention, with 3 rats in each subgroup. The rats in all groups were intervened by intranasal inoculation, and their lung tissue samples and bronchoalveolar lavage fluid (BALF) were collected from every sub-group at 0, 5, 10 and 15 days post intervention. And then, the lung index, the white blood cell count, IL-4, TNF-α, IFN-γ and the amount of pseudomonas aeruginosa (PA) in BALF were detected. And the results of different subgroups of two group were further compared. Results: After intervention, the lung index of the experimental group increased gradually with the time prolonged (from 5d to 15d), which was different to that of control group significantly (t=-2.46, t=-3.26, t=-3.73, P<0.05). The number of white blood cell in BALF of the experimental sub-group were significantly higher than those of control sub-group. And IL-4 and TNF-αin in BALF of experimental group appeared growing trend after molding intervention, and the differences of them between the two groups were significant (t=4.24, t=4.88, P<0.05), while IFN-γ was opposite. The number of PA in BALF of the experimental group appeared increasing trend with time extension after molding intervention. Conclusion: The rat pneumonia model are constructed successfully from PA by intranasal inoculation can be used in the mechanism study of prevention and treatment with traditional Chinese medicine (TCM) for pneumonia.
作者
丁军颖
桂红
高翔
崔煦然
丁雪霏
卢幼然
安世栋
洪燕英
刘清泉
DING Jun-ying;GUI Hong;GAO Xiang(Beijing Key Lab of Basic Study on Infections Diseases of Traditional Chinese Medicine, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing Institute of Traditional Chinese Medicine, Beijing 100010, China)
出处
《中国医学装备》
2019年第6期149-152,共4页
China Medical Equipment
基金
北京市自然科学基金(7182071)“基于TLR4-IRAK1-TRAF6探索芪归银对耐药PA所致肺炎微环境的调控机制”
国家自然科学基金(81503399、81373813)“基于HMGB1/RAGE/TLR2/4探索扶正透邪方对肺炎大鼠免疫失衡的逆转机制”“基于β-内酰胺酶和外膜蛋白探讨扶正透邪方对铜绿假单胞菌耐药性的作用机制”
首都医科大学附属北京中医医院院级课题(YJ-201734)“基于MAPK探索芪归银方对耐药菌致肺炎微环境的分子调控机制”
关键词
铜绿假单胞菌
滴鼻
肺炎
肺泡灌洗液
Pseudomonas aeruginosa
Intranasal inoculation
Pneumonia
Bronchoalveolar lavage fluid
