摘要
目的:观察新型抗癌复方蟾山护金方含药血清对肺腺癌A549细胞增殖的影响,并探讨其能否诱导A549细胞凋亡。方法:将30只SD大鼠随机分成3组,分别为含药血清组、消癌平组、阴性血清组,每组10只,各组依次灌胃蟾山护金方煎剂、消癌平片水溶剂和生理盐水,制备含药血清,利用含药血清做A549细胞增殖抑制实验(MTT实验)、细胞凋亡实验和检测“死亡蛋白酶”caspase-3的表达变化。结果:通过MTT实验,发现蟾山护金方含药血清能够抑制A549细胞的增殖,且呈剂量相关性,与阴性血清组比较,差异有统计学意义(P<0.05);与消癌平组比较,差异有统计学意义(P<0.05)。通过流式细胞仪检测细胞凋亡,发现蟾山护金方含药血清中浓度组和高浓度组诱导A549细胞早期凋亡,与阴性血清组比较,差异有统计学意义(P<0.05;P<0.05)。通过Western-Blot实验发现A549细胞caspase-3激活上调。结论:新型抗癌复方蟾山护金方含药血清能抑制A549细胞的增殖并诱导其凋亡,且呈剂量正相关性。
Objective: To observe the effects of new anticancer prescription of Chanshan Hujin on the proliferation of lung adenocarcinoma A549 cells,and to investigate that whether it could induce the apoptosis of A519. Methods: A total of 30 SD rats were randomly divided into three groups of a serum containing drug group,a Xiao-Ai-Ping group and a normal saline group,with 10 rats in each group.Each group was intragastric administrated successively with the Decoction of Chan-Shan-Hu-Jin Prescription,Xiao-Ai-Ping water solvent and normal saline,to prepare the serum containing drug.A549 cell proliferation inhibition test(MTT test),apoptosis assay and detection the expression of “death protease” Caspase-3 was carried out with serum containing drug. Results: Through the cell proliferation inhibition test(MTT test),it was found that the group of Chan-Shan-Hu-Jin Prescription could inhibit the proliferation of A549 cells,and had positive correlation with dose.Compared with the negative group,the difference was significant( P <0.05).The difference was significant when compared with the Xiao-Ai-Ping group( P <0.05).The apoptotic detection was carried out by flow cytometry.It was found that the early apoptosis rate after the medium concentration treatment group and high concentration treatment group,and it had significant difference when compared with the negative group( P <0.05, P <0.05).Western-Blot test showed that the activation of “death protease” caspase-3 was up-regulated. Conclusion: The serum containing drug of Chan-Shan-Hu-Jin Prescription can inhibit the proliferation and induce the apoptosis of A549 cell,and has positive correlation with dose.
作者
高强
唐源苑
钟丽娅
卢芳国
宁毅
周小青
Gao Qiang;Tang Yuanyuan;Zhong Liya;Lu Fangguo;Ning Yi;Zhou Xiaoqing(Hunan University of Chinese Medicine,Changsha 410208,China)
出处
《世界中医药》
CAS
2019年第6期1388-1392,1396,共6页
World Chinese Medicine
基金
湖南省中医药管理局课题(2015112)
湖南省教育厅课题(15C1035)
湖南省卫生计生委课题(C2017027)
湖南中医药大学课题(9982-1001-011)
湖南中医药大学校级重点学科《基础医学》资助
关键词
蟾山护金方
增殖
抑制
细胞凋亡
抗癌
肺腺癌细胞
大鼠
Prescription of Chan-Shan-Hu-Jin
Proliferation
Inhibition
Cell apoptosis
Anti-cancer
Lung adenocarcinoma
Rats
