摘要
以甘农2号小黑麦和小黑麦新品系P4为试验材料,石大1号小黑麦(CK1)和中饲1048小黑麦(CK2)为对照,测定低温胁迫(0℃、-10℃、-20℃、-30℃)供试材料的可溶性糖,脯氨酸和丙二醛含量,以及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性,以评价甘农2号小黑麦抗寒性强弱。结果表明:小黑麦材料间除P4的MDA含量(0.29μmol/g)显著高于其他3个材料外,其他指标均无显著差异;随着低温胁迫加剧,小黑麦叶片的可溶性糖在-20℃时最高,脯氨酸含量在-10℃时最高,MDA含量和SOD,POD和CAT活性均在-30℃时最高;从小黑麦材料×低温胁迫交互作用分析,抗寒性相关生理指标均存在极显著差异,在0℃和-10℃时,CK2的各项抗寒性生理指标显著高于甘农2号小黑麦和小黑麦品系P4,但随着低温胁迫程度加剧,甘农2号小黑麦和小黑麦P4的脯氨酸含量,SOD,POD,CAT活性迅速上升,甘农2号小黑麦和小黑麦品系P4表现出较强的抗寒性。总体的变化趋势为CK1的各项生理指标均低于CK2,说明CK1的抗寒性低于CK2。基于隶属函数的综合评价也同样表明,甘农2号小黑麦的抗寒性最强,其次为P4,CK1和CK2的抗寒性较弱。
The cold resistance of Triticale wittmack cv.Gannong No.2 and new line P4 was evaluated by using Triticale wittmack cv.Shida No.1 (CK 1) and Zhongsi 1048 (CK 1) as the control.Parameters including the contents of soluble sugar,proline and MDA,and activities of SOD,POD and CAT were determined at different low temperature treatments (0℃,-10℃,-20℃,-30℃).Results showed that no significant differences existed among the triticale materials except for the MDA content of P4,which was significantly higher than that of the other materials.Along with the cold stress,the contents of soluble sugar and proline in triticale leaf reached the highest at -20℃ and -10℃ respectively,but for the MDA content,activities of SOD,POD and CAT,the highest values were obtained at -30℃.Based on the interaction between triticale materials and low temperature stress days,there were significant differences in physiological indexes related to cold resistance.When the temperature varied from 0℃ to -10℃,the physiological indexes of cold resistance of CK 2 were significantly higher than Gannong No.2 and P4,but along with the cold stress,the contents of proline,SOD,POD and CAT in Gannong No.2 and P4 increased rapidly,and these two materials showed strong cold resistance.In general,all physiological indexes of CK 1 were lower than CK 2,indicating that the cold resistance of CK 1 was lower than CK 2.Comprehensive evaluation showed that Gannong No.2 had the strongest cold resistance,followed by line P4,and the cold resistance of CK 1 and CK 2 was poor.
作者
马文馨
宋谦
田新会
杜文华
MA Wen-xin;SONG Qian;TIAN Xin-hui;DU Wen-hua(College of Grassland Science,Gansu Agricultural University/Key Laboratory for Grassland Ecosystem of Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S. Centers for Grazing Land Ecosystem Sustainability,Lanzhou 730070,China)
出处
《草原与草坪》
CAS
CSCD
2019年第3期85-91,共7页
Grassland and Turf
基金
甘肃省草地畜牧业可持续发展创新团队项目(2017C-11)
国家重点研发计划(2018Y FD0502402-3)
国家自然科学基金项目(31760702)资助
关键词
小黑麦
生理生化指标
抗寒性评价
Triticale wittmack
physiological index
cold resistance evaluation
