摘要
目的通过小鼠感染MNV后外周血免疫指标的改变来评估MNV感染对小鼠免疫功能的影响。方法选取KM,BALB/c,BALB/c-nu,C57BL/6及NIH等五个品系小鼠,每个品系分成MNV感染组和对照组,于感染前(第0天)和感染后的第7、14、21、28天从眼周静脉采集抗凝血,流式细胞术测定总T细胞、CD4^+T细胞、CD8^+T细胞、总B细胞、NK细胞及NK-T细胞占总淋巴细胞的百分比及干扰素、白介素、趋化因子等13种细胞因子的含量,比较感染后各指标总体均值的差异,分析各时间点变化情况。结果与对照组相比,KM小鼠感染组总T细胞、CD4^+T细胞含量显著增高(P<0.01),CD4/CD8显著增高(P<0.05),总B细胞及CXCL10含量显著降低(P<0.01);BALB/c小鼠CD8^+T细胞显著降低(P<0.05),IFN-γ含量显著增高(P<0.01);BALB/c-nu小鼠各免疫细胞均无统计学差异,TNF-α、CXCL1及CXCL10显著增高(P<0.01),CCL2显著增高(P<0.05),IFN-β显著降低(P<0.05);C57BL/6小鼠CD4^+(P<0.05)CD8^+T细胞(P<0.01)均显著降低,总B细胞及IL-10显著增高(P<0.05),CCL5及GM-CSF显著降低(P<0.05);NIH小鼠总T细胞和CD8^+T细胞显著增高(P<0.01),CD4/CD8显著降低(P<0.05),总B细胞显著降低(P<0.01),细胞因子均无统计学差异。对于有统计学差异的免疫指标,有半数感染第7天即出现显著差异,大多数第28天仍存在显著差异。结论MNV的感染会影响正常小鼠的细胞免疫和体液免疫应答,而且不同品系的动物影响不同,建议在进行免疫相关的动物实验时选择MNV阴性小鼠,以避免实验结果出现偏差。
Objective To evaluate the effect of murine norovirus(MNV)infection on immune function in mice by assessing the changes in peripheral blood immune parameters.Methods KM,BALB/c,BALB/c-nu,C57BL/6,and NIH mice were selected.Each strain was divided into an MNV infection group and a control group.Anticoagulated blood samples were collected from the periocular vein plexus before infection(day 0)and at 7,14,21,and 28 d post-infection.Flow cytometry was used to determine the percentage of total T cells,CD4^+T cells,CD8^+T cells,total B cells,NK cells and NK-T cells in the total lymphocytes and the levels of 13 cytokines such as interferon and interleukin,and chemokines.To compare the differences in the mean values of each index after infection,the changes at each time point were analyzed.Results Compared with the control group,the level of total T cells and CD4^+T cells in the infected group of KM mice significantly increased(P<0.01),CD4/CD8 significantly increased(P<0.05),and the total B cell and CXCL10 content significantly decreased(P<0.01).The level of CD8^+T cells in infected BALB/c mice significantly decreased(P<0.05),and IFN-γlevels significantly increased(P<0.01).There was no significant difference between the levels of immune cells in infected and control BALB/c-nu mice,although in the infected BALB/c-nu mice TNF-α,CXCL1 and CXCL10 significantly increased(P<0.01),CCL2 significantly increased(P<0.05),and IFN-βsignificantly decreased(P<0.05).CD4^+(P<0.05)and CD8+T cells(P<0.01)in the infected C57BL/6 mice significantly decreased,total B cells and IL-10 significantly increased(P<0.05),and CCL5 and GM-CSF significantly decreased(P<0.05).Total T cells and CD8^+T cells in the infected NIH mice significantly increased(P<0.01),CD4/CD8 significantly decreased(P<0.05),and total B cells significantly decreased(P<0.01),while there were no significant differences in cytokine levels.In terms of statistically significant changes in immune indicators,half of all immune indicators showed significant differences on the 7th day of infection,and most still demonstrated significant differences on the 28th day.Conclusions MNV infection affects cellular and humoral immune responses in otherwise healthy mice,and different mouse strains demonstrate different effects.Researchers should select MNV-negative mice in immunologically relevant animal experiments to avoid bias in the experimental results.
作者
李晓波
付瑞
王吉
王淑菁
李威
王莎莎
秦骁
黄宗文
贺争鸣
岳秉飞
赵德明
LI Xiaobo;FU Rui;WANG Ji;WANG Shujing;LI Wei;WANG Shasha;QIN Xiao;HUANG Zongwen;HE Zhengming;YUE Bingfei;ZHAO Deming(College of Veterinary Medicine,China Agricultural University,Beijing 100094,China;Laboratory Animal Institute,National Institutes for Food and Drug Control,Beijing 100050)
出处
《中国比较医学杂志》
CAS
北大核心
2019年第7期67-75,共9页
Chinese Journal of Comparative Medicine
基金
国家科技支撑计划项目(2015BAI09B00)
关键词
小鼠诺如病毒
品系
外周血
免疫功能
小鼠
murine norovirus
strain
peripheral blood
immune function
mouse
