摘要
目的探讨经血来源干细胞(MenSCs)移植对多囊卵巢综合征(PCOS)大鼠模型的治疗效果。方法成年雌性SD大鼠45只均分为3组(n=15):对照组不作处理;PCOS组肌内注射脱氢表雄酮(DHEA,60mg/kg)诱导PCOS;移植组肌内注射DHEA,饲养3周后后经尾静脉注射MenSCs悬液。各组饲养5周后处死,取静脉血,酶联免疫吸附试验(ELISA)检测促卵泡激素(FSH)、黄体生成素(LH)、雌二醇(E2)、孕激素(P)、睾酮(T)、泌乳素(PRL)水平。取卵巢组织进行HE染色观察卵巢形态。实时荧光定量检测卵巢组织miRNA-135表达。Westernblot检测卵巢组织Bax和Bcl-2蛋白表达。结果相对于对照组,PCOS组的FSH降低,LH和T均升高(P<0.05);相对于PCOS组,移植组的FSH升高,LH和T均降低(P<0.05)。对照组卵泡形态正常,PCOS组卵巢可见较多囊状扩张的卵泡,移植组多囊状扩张的卵泡较PCOS组减少。与对照组比较,PCOS组miRNA-135和Bcl-2蛋白相对表达水平降低,而Bax蛋白相对表达水平升高(P<0.05);与PCOS组比较,移植组miRNA-135和Bcl-2蛋白相对表达水平升高,Bax蛋白相对表达水平降低(P<0.05)。结论经血来源干细胞移植对大鼠PCOS有明显的改善作用,其机制可能通过上调miRNA-135的表达,进而调控Bax/Bcl-2途径来实现。
Objective To investigate the therapeutic effect of transplantation of menstrual stem cells (MenSCs) on rat model of polycystic ovary syndrome (PCOS). Methods A total of 45 female SD rats were divided into 3 groups. The control group was not treated. The PCOS group was intramuscularly injected with dehydroepiandrosterone (DHEA, 60 mg/kg) to induce PCOS. The transplant group was intramuscularly injected with DHEA. After 3 weeks, the MenSCs suspension was injected through the tail vein. After 5 weeks of feeding, venous blood was taken. ELISA kit was used to detect follicle- stimulating hormone (FSH), luteinising hormone (LH), estradiol (E2), progesterone (P), testosterone (T) and prolactinemia (PRL) levels. Ovarian tissues were taken for HE staining to observe ovarian morphology. qPCR was used to detect miRNA- 135 expression in ovarian tissue. Western blot assay was used to detect the expression of Bax and Bcl-2 proteins in ovarian tissue. Results The FSH level was significantly decreased, and LH and T levels were significantly increased, in the PCOS group than those in the control group (P<0.05). The FSH level was significantly increased, and LH and T levels were significantly decreased in the transplantation group than those in the PCOS group (P<0.05). The follicle morphology was normal in the control group. Follicles with more cystic dilatation in ovary were observed in the PCOS group. The follicles with polycystic expansion were less in the transplantation group than those in the PCOS group. The expressions of miRNA-135 and Bcl-2 protein were significantly decreased in the PCOS group than those in the control group, and the expression of Bcl- 2 protein was significantly higher in the PCOS group than that in the control group (P<0.05). The expressions of miRNA- 135 and Bcl-2 protein were significantly higher, the expression of Bas was significantly decreased, in the transplantation group than those of PCOS group (P<0 .05). Conclusion MenSCs has a significant improvement effect on rat PCOS, which may be achieved by up-regulating the expression of miRNA-135 and then regulating the Bax/Bcl-2 pathway.
作者
刘荣华
何文容
LIU Rong-hua;HE Wen-rong(Color Doppler,first affiliated Hospital of Yangtze River University,434000;Department of Obstetrics and Gynaecology,First Affiliated Hospital of Changjiang University,434000)
出处
《天津医药》
CAS
北大核心
2019年第7期709-712,共4页
Tianjin Medical Journal
