摘要
重组酶聚合酶等温扩增技术(recombinase polymerase amplification,RPA)是一种新型核酸等温扩增技术。该技术相对于PCR等其他核酸体外扩增技术具有灵敏度高、特异性强、检测时间短、操作简便等特点。RPA技术在常温下即可进行等温扩增,其最适温度在37~42℃,RPA技术可在简单设备甚至恶劣环境对核酸的进行快速扩增,结合侧流层析技术荧光检测装置可对检测结果进行定性定量分析。文章综述了RPA技术的原理,操作条件及其在食源性病毒、食源性致病菌、动物源性检测及转基因食品检测等方面的应用,为该技术进行更加深入和广泛的研究提供参考。
Recombinase-polymerase amplification(RPA)is a new isothermal amplification technique for detecting specific nucleotide sequences.Compared with regular PCR amplification,it has advantages of high sensitivity,high specificity,short detection time and simple operation.At optimal temperature(37-42℃),RPA can be used to amplify specific nucleotide sequences rapidly with simple equipment even in harsh environment.The detection results can be qualitatively and quantitatively analyzed by combining with fluorescence detection device of side-flow chromatography technology.The principles and operating conditions of RPA as well as its applications in detecting food-borne virus,pathogenic bacteria,animal-derived components and transgenic foods were summarized in this paper in order to provide a reference for further extensive researches on RPA.
作者
兰海鸥
柯义强
马咸莹
程浩
丁功涛
李明生
陈士恩
马忠仁
魏嘉
LAN Haiou;KE Yiqiang;MA Xianying;CHENG Hao;DING Gongtao;LI Mingsheng;CHEN Shien;MA Zhongren;WEI Jia(China-Malaysia National Joint Laboratory,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,China;College of Life Sciences and Engineering,Northwest Minzu University,Lanzhou 730030,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2019年第14期233-238,共6页
Food and Fermentation Industries
基金
中国-马来西亚清真食品国家联合实验室,科技部援外项目(KY201501005)
中央高校基本科研业务费专项资金项目:利用RPA技术快速检测食品中花生过敏原方法的建立(31920180127)
西北民族大学双一流和特色发展引导专项(10018703,1001070204)
教育部“长江学者和创新团队发展计划”项目(IRT_17R88)
甘肃省科技计划资助(17YF1WA166)
