摘要
为了筛选用于淡水环境镉污染监测与评估的生物标志物,研究用2.698mg/LCd^2+暴露背角无齿蚌7,14,21,28d,检测其鳃和消化腺中7种生物标志物的变化,包括超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽硫转移酶(GST)、谷胱甘肽(GSH)、总抗氧化能力(T-AOC)和脂质过氧化产物丙二醛(MDA)。结果表明,背角无齿蚌经Cd^2+暴露后,鳃MDA含量显著增高,SOD、CAT和GPx活性受到抑制,GST和GSH代偿性地发挥了抗氧化作用,T-AOC显著增高;消化腺MDA含量无显著变化,SOD、CAT、GPx、GST和GSH均受到诱导,共同应对Cd^2+暴露导致的氧化损伤,T-AOC显著增高,但在28d略有降低。研究表明,背角无齿蚌消化腺比鳃的抗氧化能力更强,受到的氧化损伤也更小;鳃SOD和CAT对Cd^2+暴露的敏感性和指示性最强,消化腺CAT在Cd^2+暴露中后期、GSH在Cd^2+暴露中前期的指示性较好。因此,其可用作监测和评估水体Cd污染的生物标志物。
To select the biomarkers for the aquatic environmental heavy metal,cadmium(Cd)especially,pollution monitor and assessment,in this study the freshwater mussel Anodonta woodiana was exposed to Cd^2+(2.698 mg/L)for 7,14,21,28 d.After exposure to Cd^2+,the 7 kinds of biomarkers which were commonly used for environmental monitor in gills and digestive gland of Anodonta woodiana were determined and analyzed,and they were superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GPx)and glutathione-s-transferase(GST),glutathione(GSH),total antioxidant capacity(T-AOC)and lipid peroxidation product malondialdehyde(MDA).The result showed that in the gills,the MDA content significantly increased,the activities of SOD,CAT,GPx were inhibited,and the GST activity and GSH content increased to compensate antioxidant defenses,and T-AOC increased significantly.In the digestive gland,there was no significant change in MDA content.SOD,CAT,GPx,GST activities and GSH content were all induced to cope with the oxidative stress from Cd^2+.The T-AOC significantly increased,but decreased slightly at 28 d.The digestive gland owned stronger antioxidant capacity and gained less oxidative damage compared to the gills.What's more,the SOD and CAT in the gills had strong sensitivity and indication to Cd^2+exposure.And in the digestive gland,CAT could indicate in the middle and late stages of Cd^2+exposure,and GSH could indicate in the middle and early stages of Cd2+exposure.Thus,SOD and CAT in the gills,CAT and GSH in the digestive gland of Anodonta woodiana can be used as biomarkers for monitoring and assessing cadmium pollution in aquatic environment.
作者
井维鑫
林子根
郎朗
刘娜
王茜
王兰
JING Weixin;LIN Zigen;LANG Lang;LIU Na;WANG Qian;WANG Lan(College of Life Science,Shanxi University,Taiyuan 030006,China)
出处
《山西农业科学》
2019年第8期1334-1340,共7页
Journal of Shanxi Agricultural Sciences
基金
山西省重点研发计划项目(201703D221008-3)
山西省回国留学人员重点科研资助项目(2016-1重点)
山西省高等学校特色重点学科建设项目(2011-SXDX-SWX-003)
关键词
背角无齿蚌
抗氧化酶
氧化应激
生物标志物
镉
Anodonta woodiana
antioxidant enzymes
oxidative stress
biomarkers
cadmium
