摘要
建立了一种直接进样快速测定环境水体中N,N-二甲基甲酰胺的超高效液相色谱方法,将水样经过0.22μm的滤头过滤后,以乙腈-水溶液为流动相,分别研究了检测波长、流动相组成、柱温和流速等实验条件对色谱分析的影响。结果表明,当检测波长196nm,流动相组成为15%的乙腈水溶液,流速0.2mL/min,柱温30℃,DMF的响应高,峰形好,使用该方法分析水体中N,N-二甲基甲酰胺的线性关系良好(R≥0.9999),加标回收率73.5%~113%,精密度2.03%~3.76%,方法检出限和测定下限分别是0.025mg/L和0.1mg/L。
A method for rapid determination of DMF in water was established by direct injection and UPLC with PDAD.After the water was filtered by a 0.22 μm filter.When acetonitrile - water was used as the mobile phase.The influence of chromatographic analysis was studied by different experimental conditions ,such as detection wavelength, the mobile phase, the flow rate and the column temperature.The optimum chromatogram conditions are the mixture mobile phase of acetonitrile - water ( 15∶ 85,v /v) and the flow rate 0.2 mL /min, the column temperature 30 ℃ and detection wavelength 196 nm.Under the optimized conditions,DMF get high response and good peak shape, the linearity of DMF is very good( R≥0.999 9).The recoveries for different samples ranged from 73.5% to 113%.RSDs were 2.03%to 3.76%.The method detection limit and minimum quantitative detection limit were 0.025 mg /L and 0.1 mg /L,respectively.
作者
乔支卫
谌衍山
张君辉
陈小军
郭荣福
QIAO Zhiwei;CHEN Yanshan;ZHANG Junhui;CHEN Xiaojun;GUO Rongfu(Environmental Monitoring Centre of Jiangxi Province,330077,Nanchang,PRC;Bureau of Ecological Environment In Guangfeng County,334600,Shangrao,Jiangxi,PRC;Ganzhou Environmental Monitoring Station,341000,Ganzhou,Jiangxi,PRC;Jiangxi Tengding Technology Co.Ltd,330077,Nanchang,PRC)
出处
《江西科学》
2019年第4期487-490,共4页
Jiangxi Science
基金
生态环境部项目“土壤环境监测技术规范(HJ/T 166-2004)”(编号:2018-6)
