摘要
为了提高麦秆转化为乙醇的效率,通过正交试验对麦秆在乙醇/水中的盐酸预处理条件和乙醇/水中盐酸预处理的麦秆同步糖化发酵条件进行了优化.结果表明:乙醇/水中麦秆盐酸预处理影响的主次顺序为盐酸质量分数、时间、温度、乙醇体积分数和底物质量浓度.乙醇/水中麦秆的最佳盐酸预处理条件为温度120℃、盐酸质量分数8%、时间60min、底物质量浓度2.5g/L和乙醇体积分数40%,此时总还原糖浓度为469.18mg/g.乙醇/水中盐酸对麦秆的预处理效果比水中的要好.乙醇/水中盐酸预处理的麦秆同步糖化发酵影响的主次顺序为酵母接种量、酶浓度、时间和温度.乙醇/水中盐酸预处理的麦秆最佳同步糖化发酵条件为温度33℃、酵母接种量0.3%、酶质量浓度0.8g/L和发酵时间4d,此时乙醇的质量浓度为22.38g/L.乙醇/水中盐酸预处理的麦秆同步糖化发酵制乙醇的效果比水中的要好.
In order to increase the efficiency of converting wheat straw into ethanol,the conditions of hydrochloric acid(HCl) pretreatment of wheat straw in ethanol/water and simultaneous saccharification and fermentation(SSF) of wheat straw pretreated by HCl in ethanol/water were optimized by orthogonal experiment.The results showed that the primary and secondary that effected HCl pretreatment of wheat straw in ethanol/water were HCl concentration,time,temperature,ethanol volume percent and substrate mass concentration.The optimal HCl pretreatment conditions of wheat straw in ethanol/water were temperature of 120 ℃,HCl concentration of 8%,time of 60 min,substrate mass concentration of 2.5 g/L and ethanol volume percent of 40%.The total reducing sugar concentration was 469.18 mg/g at the optimal pretreatment conditions.The effect of HCl pretreatment of wheat straw in ethanol/water solvent was better than that in water.The primary and secondary that effected the SSF of wheat straw pretreated by HCl in ethanol/water was yeast inoculation,enzyme concentration,time and temperature.The optimal SSF conditions of wheat straw pretreated by HCl in ethanol/water were temperature of 33 ℃,yeast inoculation of 0.3%,enzyme concentration of 0.8 g/L and fermentation time of 4 d.The ethanol concentration was 22.38 g/L at the optimal SSF conditions.The effect of SSF of wheat straw pretreated by HCl in ethanol/water solvent to ethanol is better than that of water.
作者
曹拢财
叶艳艳
王佳琪
胡银平
付艺博
祝勇
崔茂金
CAO Longcai;YE Yanyan;WANG Jiaqi;HU Yinping;FU Yibo;ZHU Yong;CUI Maojin(School of Chemistry and Chemical Engineering,Henan Institute of Science and Technology,Xinxiang 453003,China)
出处
《河南科技学院学报（自然科学版）》
2019年第4期36-40,共5页
Journal of Henan Institute of Science and Technology(Natural Science Edition)
基金
河南科技学院大学生创新训练项目(2018CX64)
河南科技学院博士科研启动基金(210010611001)
关键词
麦秆
有机溶剂
酸预处理
同步糖化发酵
正交试验
wheat straw
organic solvent
acid pretreatment
simultaneous saccharification and fermentation
orthogonal experiment
