摘要
目的:为提升保健食品补骨胶囊的质量标准,采用不同前处理方法对钙含量测定影响进行对比分析研究,确定最佳的补骨胶囊钙含量测定前处理方法。方法:样品采用微波消解法、湿法消解、干法灰化三种前处理方法,以火焰原子吸收光谱法对处理后的样品进行钙含量测定,对三种方法处理后测得的结果进行对比研究,比较不同前处理方法对钙含量测定结果的精密度、重复性,选取最佳补骨胶囊样品前处理方法。结果:火焰原子吸收光谱法测定钙在0~6.00 mg/L范围内线性关系良好,回归方程为y=0.0289x-0.00005,r=1;微波消解处理补骨胶囊样品的重复性较湿法消解、干法灰化的好;微波消解加样回收率限度在95%~102%之间,平均回收率为99%(RSD为2.27%,n=6)。结论:采用微波消解作为样品前处理方法,测定补骨胶囊钙含量结果相对稳定,表明其方法稳定可行,可有效控制补骨胶囊的质量。
Objective In order to improve the quality standard of health food Bugu capsules,theeffects of different pretreatment methods on the determination of calcium content were compared and studied,and the best pretreatment method for the determination of calcium content in Bugu capsules was determined.Method The samples were treated by microwave digestion、wet digestion and dry ashing.The calcium content of the treated samples was determined by Flame Atomic Absorption Spectrometry,and the results of the three methods were compared.The precision and reproducibility of different pretreatment methods for the determination of calcium content were compared,and the best pretreatment method of Bugu capsule samples was selected.Result The good linear range of calcium determined by Flame Atomic Absorption Spectrometry was 0~0.6 mɡ/L,the regression equation was y=0.029x+0.0002,r=0.9999;The reproducibility of microwave digestion was better than that of wet digestion and dry ashing;The recovery limit of microwave digestion was between 95%~102%,and the average recovery was 99%(RSD is 2.27%,n=6).Conclusion The method is simple,accurate,reproducibility,high sensitivity,stable and feasible.It is suitable for the determination of naringin in Bugu capsules and can better control the quality of its drugs.
作者
王亮
谢宇
罗君
许乾丽
薛鑫宇
张丽艳
WANG Liang;XIE Yu;LUO Jun;XU Qianli;XUE Xinyu;ZHANG Liyan(Guiyang Collage of Traditional Chinese Medicine,Guiyang 550025,China;Guizhou Guangjitang Pharmaceutical Company Limited,Guiyang 550014,China;First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine (TCM),Guiyang 550001,China;Guizhou Institute for Food and Drug Control,Guiyang 550004,China)
出处
《中国民族民间医药》
2019年第16期36-39,共4页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
贵州省国内一流建设学科(中药学)(GNYL[2017]008号)
贵州省科技支撑计划项目(黔科合支撑[2018]2772)
贵州省科技厅贵阳中医学院第一附属医院联合基金(黔科合LH字[2015]7801)
国家中医药管理局中药特色技术传承人才项目(国中医药办人教函[2018]204号)
贵阳国家高新技术产业开发区管理委员会科技型中小企业技术创新基金(GXCX2015-012)
关键词
补骨胶囊
钙
含量测定
火焰原子吸收光谱法
Bugu Capsule
Calcium
Content Determination
Flame Atomic Absorption Spectrometry
