摘要
红冬孢酵母(Rhodosporidium)是一种天然的微生物油脂和β-类胡萝卜素高产菌,有望被开发为工业平台菌株。近年来,基因组测序完成和各种组学研究的开展,为红冬孢酵母分子遗传操作技术开发奠定了基础。基因元件(如启动子、终止子、报告基因和筛选标记等)的挖掘和引入,基因转化方法开发以及基因操纵技术(如基因失活和过表达)等的完善,加速了红冬孢酵母的代谢工程研究进程。本文中,笔者总结了红冬孢酵母在分子遗传操作技术方面的研究进展,力图勾勒出红冬孢酵母的分子遗传操作技术研究的概貌,为后续研究提供借鉴。
Rhodosporidium is a promising industrial platform strain.It natively produces microbial oil and β-carotenoid with high titer and yield.Recently,great progress has been made in genome sequencing and multi-omics study,providing the basis to develop molecular genetic manipulation technology.Characterization and application of genetic components or elements (such as promoters,terminator,reporter genes and selective markers),development of gene transformation methods,and improvement of genetic manipulation technology (gene inactivation and overexpression) have accelerated the metabolic engineering of Rhodosporidium.Here,we summarize the research progress in molecular genetic manipulation technology of Rhodosporidium.We also outline the research framework of molecular genetic manipulation of Rhodosporidium to provide reference for future research.
作者
马冬石
陈震
彭毅雯
闻志强
金明杰
MA Dongshi;CHEN Zhen;PENG Yiwen;WEN Zhiqiang;JIN Mingjie(School of Environmental and Biological Engineering,Nanjing University of Science and Technology,Nanjing 210094,China)
出处
《生物加工过程》
CAS
2019年第5期504-508,共5页
Chinese Journal of Bioprocess Engineering
基金
国家重点研发计划政府间合作项目(2016YFE0105400)
江苏省杰出青年基金(BK20170037)
国家自然科学基金(21706133)
中央高校基本科研业务费(30918011310)
关键词
红冬孢酵母
分子遗传操作
基因元件
基因转化
代谢工程
Rhodosporidium
molecular genetic manipulation
gene elements
gene transformation
metabolic engineering
